Angiotensin converting enzyme 2 improves insulin secretion in islets

doi:10.3969/j.issn.1006-7795.2017.02.002

Abstract

Abstract: Objective The aim of the study was to identify the roles of angiotensin-converting enzyme 2 (ACE2) in insulin secretion in islets and to characterize the related mechanisms. Methods Islets from C57BL/6 mice were treated with palmitate acid and infected with adenovirus combined ACE2 or GFP. Glucose stimulated insulin secretion was assayed by ELISA kit. Reactive oxygen species (ROS) was measured by dihydroethidium (DHE). The expression of glucose metabolism gene expression and caspase was assayed by Western blotting. Results ①ACE2 over-expression increased insulin secretion. ②ACE2 over-expression down-regulated ROS content in islets. ③ACE2 over-expression down-regulated apoptosis in islets. Conclusion ACE2 could be involved in improving insulin secretion via anti-oxidative and anti-apoptotic effects.

Key words: rennin-angiotensin system, angiotensin-converting enzyme 2, insulin, oxidative stress, apoptosis

CLC Number:

R587.1

Shi Tingting, Yang Fangyuan, Cao Xi, Lu Jing, Xie Rongrong, Yuan Mingxia, Yang Jinkui. Angiotensin converting enzyme 2 improves insulin secretion in islets[J]. Journal of Capital Medical University, 2017, 38(2): 151-155.

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URL: https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/10.3969/j.issn.1006-7795.2017.02.002

https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/Y2017/V38/I2/151

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