Table of Content

21 March 2017, Volume 38 Issue 2

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Progresses in studies on novel biomarkers for early diagnosis of diabetic nephropathy

Song Lini, Liu Jingyi, Zhang Yichen, Cao Xi, Yuan Mingxia, Yang Jinkui

2017, 38(2):  145-150.  doi:10.3969/j.issn.1006-7795.2017.02.001

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Diabetic kidney disease (DKD) as a common and severe chronic complication of type 2 diabetes mellitus (T2DM) has rapidly become an important public health problem. It can be reversed in early stage after treatment, or become end-stage renal disease in advanced stage. Therefore, early diagnosis and prediction of DKD for clinical treatment is particularly important. Although microalbuminuria (MAU) is an early marker for kidney damage in type 2 diabetes mellitus, its predictive value is now questioned. In recent years, it was found that proteomics may become a new tool in the diagnosis of diabetic nephropathy. Haptoglobin (HP) as an acute phase protein with a variety of functions was found may be closely related to the occurrence and development of diabetic nephropathy. Therefore, this article reviewed the progress on research of haptoglobin with the pathogenesis of diabetic nephropathy.

Angiotensin converting enzyme 2 improves insulin secretion in islets

Shi Tingting, Yang Fangyuan, Cao Xi, Lu Jing, Xie Rongrong, Yuan Mingxia, Yang Jinkui

2017, 38(2):  151-155.  doi:10.3969/j.issn.1006-7795.2017.02.002

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Objective The aim of the study was to identify the roles of angiotensin-converting enzyme 2 (ACE2) in insulin secretion in islets and to characterize the related mechanisms. Methods Islets from C57BL/6 mice were treated with palmitate acid and infected with adenovirus combined ACE2 or GFP. Glucose stimulated insulin secretion was assayed by ELISA kit. Reactive oxygen species (ROS) was measured by dihydroethidium (DHE). The expression of glucose metabolism gene expression and caspase was assayed by Western blotting. Results ①ACE2 over-expression increased insulin secretion. ②ACE2 over-expression down-regulated ROS content in islets. ③ACE2 over-expression down-regulated apoptosis in islets. Conclusion ACE2 could be involved in improving insulin secretion via anti-oxidative and anti-apoptotic effects.

Human ether-α-go-go related gene channels improves glucose metabolism via anti-oxidative stress in HepG2 cells

Lu Jing, Sheng Han, Cheng Cheng, Song Lini, Zhang Yichen, Xie Rongrong, Yuan Mingxia, Yang Jinkui

2017, 38(2):  156-160.  doi:10.3969/j.issn.1006-7795.2017.02.003

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Objective To evaluate the effect of the new pathway of human ether-α-go-go related gene(hERG) channels on glucose metabolism in hepatic cells. Methods Liver hepatocellular carcinoma (HepG2) cells were transfected with hERG channels over-expression plasmid DNA. pcDNA3. 1-GFP plasmid was used as a negative control. The expression of hERG, phosphoenolpyruvate carboxykinase(PEPCK) and glucose-6-phosphatase (G6Pase) were detected by Western blotting. The mRNA level of glucose transporter type 2 (GLUT2), insulin receptor substrate (IRS-2), adenosine 5'-monophosphate(AMP)-activated protein kinase (AMPKα2), p22, p47, p67 and p91 were observed by real-time PCR(RT-PCR). Results The experiments showed that hERG gene can be expressed in HepG2 cells. Over-expression of hERG gene in HepG2 cells could down-regulate the expression of PEPCK, G6Pase. while GLUT2, IRS-2, AMPKα2 genes were up-regulated. Over-expression of hERG gene also inhibited the relative mRNA level of p22, p47, p67and p91. Conclusion hERG channels could be involved in improving glucose metabolism via anti-oxidative effects.

Effect of KCN potassium channel gene on lipid metabolism in mice

Liu Jingyi, Lu Jing, Yang Jinkui

2017, 38(2):  161-166.  doi:10.3969/j.issn.1006-7795.2017.02.004

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Objective To study the effect of potassium channel gene (KCN) on lipid metabolism in mice and its related signal pathway and to provide a new therapeutic target for dyslipidemia in diabetes. Methods Our group constructed KCN channel knock-out mouse model. In this study, KCN channel knock-out mice and control C57BL/6 mice will be examined. We will detect total cholesterol, triglyceride, high-density lipoprotein and low-density lipoprotein in their serum, the protein level of adenosine 5'-monophosphate (AMP)-activated protein kinase (AMPK) pathway from rat's tissue was detected by western blot and the level of hepatic steatosis will be observed by HE staining. Results The serum levels of total cholesterol, triglyceride and low-density lipoprotein in KCN gene knock-out mouse were all significantly higher than those in controls, and high-density lipoprotein is lower than control (P<0.05). The results of Western blotting showed that phosphate-protein kinase B (Phos-AKT) and phos-AMPK levels in liver tissue of KCN gene knockout mice were lower than those in normal control group, and phosphate-acetyl CoA carboxylase (phos-ACCα) level was significantly higher (P<0.05). Conclusion KCN channel influences lipid metabolism in mice by classical fatty acid oxidation AMPK pathway.

Correlation between serum vitamin D level and thyroid function and clinical characteristics in the newly-diagnosed type 2 diabetes mellitus patients

Cui Limei, Liu Cuiping, Fu Hanjing, Yan Yehua, Zheng Xiaomin, Tao Jing, Feng Jianping, Yuan Mingxia, Yang Jinkui

2017, 38(2):  167-171.  doi:10.3969/j.issn.1006-7795.2017.02.005

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Objective To observe relation between serum vitamin D level and thyroid function, clinical characteristics in the newly-diagnosed type 2 diabetes mellitus(T2DM) patients.Methods A total of 239 patients with the newly-diagnosed T2DM were selected in Department of Endocrinology, Beijing Chuiyangliu Hospital from May 2015 to October 2016. According to serum 25-hydroxyvitamin D[25(OH)D] level, these patients were divided into four groups:normal group ≥30 ng/mL, insufficiency group 20 ng/mL≤25(OH)D<30 ng/mL, deficiency group 10 ng/mL≤25(OH)D<20 ng/mL and severe deficiency group<10 ng/mL. Clinical characteristics and thyroid function were compared in these groups.Results The incidence of serum 25(OH)D deficiency was 56.9% in the newly-diagnosed T2DM group.There were significant differences in age, glycosylated hemoglobin (HbA1c), thyroid stimulating hormone (TSH) in these groups (P<0.05).The linear regression analysis showed that serum calcium (Ca), parathyroid hormone (PTH) were positively correlated with serum 25(OH)D (P<0.05), but gender, age, smoking, HbA1c, TSH were negatively correlated with serum 25(OH)D (P<0.05).Conclusion Female, age, smoke, HbA1c, TSH are important risk factors for deficiency of serum 25(OH)D in the newly-diagnosed T2DM patients.

Correlation between serum vitamin D level and thyroid function and clinical characteristics in the newly-diagnosed type 2 diabetes mellitus patients

Ma Namin, Wang Min, Nie Yuanyuan, Yu Shilin, Geng Yahui, Dong Jianquan, Yuan Meiying, Yang Jinkui

2017, 38(2):  172-177.  doi:10.3969/j.issn.1006-7795.2017.02.006

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Objective To investigate the changes of serum thyroid hormone in elderly patients with type 2 diabetes mellitus (T2DM) and relevant factors. Methods According to the random number method,140 elderly patients with T2DM admitted into our hospital were assigned to the case group, and 147 healthy persons without diabetes in the same period were assigned to the control group. The thyroid-stimulating hormone(TSH),triiodothyronine(T3), thyronine (T4),anti-thyroid peroxidase antibody (TPOAb), anti-thyroglobulin antibody (TGAb) and thyroid-stimulating hormone receptor antibodies(TRAb) were assayed. Thyroid color Dopplar ultrasonography was performed. Fasting plasma glucose (FPG), glycosylated hemoglobin A1c (HbA1c) were additionally given for T2DM patients. The patients were divided into several groups according to their age、duration、FPG and HbA1c levels.The difference of thyroid hormone levels in each group and impact factors to thyroid hormones in T2DM patents were analyzed. Results ①Compared with control group, the level of T3 in the case group were significantly lower(P<0.05). ②With the increase of age、duration of diabetes、FPG and HbA1c level in the erlderly patients with diabetes,the level of T3 were significantly decreased(P<0.05).③In the case group, T3 was negatively correlated with age, duration of diabetes, FPG and HbA1c. Duration of diabetes and HbA1c were the independent factors influencing the changes of T3. Conclusion Thyroid hormones level in elderly patients with T2DM reflects the degree of glucose metabolism disorder.

Prepulse inhibition and the related factors among healthy people and patients with chronic schizophrenia

Fan Yu, Yang Ningbo, Tian Qing, Zhu Xiuqiang, Wang Chuanyue

2017, 38(2):  178-185.  doi:10.3969/j.issn.1006-7795.2017.02.007

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Objective To study the prepulse inhibition (PPI) and its related factors among healthy people and patients with chronic schizophrenia. Methods The PPI and cognitive performance (repeatable battery for the assessment of neuropsychological status (RBANS) and Stroop Color-world test) were tested in 50 healthy people (HP)and 75 chronic schizophrenia patients (SCZ). Positive and negative syndrome scale (PANSS) was used to evaluate the psychotic symptoms of patients. Results (1) Compared with HP, SCZ showed decreased PPI% (9.4%±29.3% vs 31.7%±26.2%,P<0.001), and the difference between two groups was found in cognitive performance (RBANS scores and its scores of all items in RBANS, Stroop color and word time). (2) Related factors of PPI% in healthy people:univariate analysis in PPI% showed that gender (male vs female, P<0.05), smoking (r=0.29,P<0.05), RBANS scores (r=0.30,P<0.05) and attention item scores (r=0.29,P<0.05) on the impact of the PPI% were statistically significant. Furthermore, multivariate ridge regression showed the same results as well as univariate analysis (ridge parameter k=2.95, adjusted R²=0.33, P<0.05). (3) Related factors of PPI% in people with chronic schizophrenia:PPI% was significantly correlated with smoking (r=0.23,P<0.05), relapses (>3 times vs≤3 times, P<0.05) in univariate analysis. And mutlivariate ridge regression showed the significantly related factors of PPI% in schizophrenia patients were gender, smoking and relapses (ridge parameter k=2.58, adjusted R²=0.26, P<0.05). Conclusion PPI% is an indicator of sensory gating, and its related factors may be different for different groups. Female and smoking may be protective factors for sensory gating in schizophrenia, more recurrence may be destroy the sensory gating function of patients with schizophrenia.

Quality of life and related factors in patients with major depressive disorder

Li Feng, Bo Qijing, Zhao Yan, Liu Hui, Wang Chuanyue

2017, 38(2):  186-191.  doi:10.3969/j.issn.1006-7795.2017.02.008

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Objective To evaluate the quality of life in patients with major depressive disorder and analyze the influencing factors. Methods A total of 92 adult patients with major depressive disorder (MDD) diagnosed according to the Diagnostic and Statistical Manual of Mental Disorders 4th edition (DSM-IV) and 80 healthy controls were evaluated using the World Health Organization Quality of Life Scale Brief (WHOQOL-BREF), the 17-item Hamilton Depression Rating Scale (HAMD-17), Hamilton anxiety scale (HAMA) and social support rating scale (SSRS) for evaluation. Results The WHOQOL-BREF dimensions scores of patients with major depressive disorder were lower than healthy controls (P<0.05); and significantly negatively correlated with HAMD-17, HAMA score; and SSRS scores were significantly correlated (P<0.01);Gender was negatively correlated with the social relationship in WHOQOL-BREF (P<0.05), while the duration was negatively correlated with the environmental areas in WHOQOL-BREF(P<0.05).Regression analysis found that HAMD-17 and physiological, psychological domain scores were negatively correlated (P<0.05). The factor of subjective score in SSRS and psychological, social relationship, environmental areas in WHOQOL-BREF were related (P<0.05); the use of the psychological field support was also affected (P<0.01). Conclusion The quality of life in patients with major depressive disorder was lower than the general population. More severe symptoms,longer durations and weaker social support,and male may result in poorer quality of life.A stable marriage helps to improve the quality of life.

An experimental study of self-face recognition speed in patients with depersonalization disorder

Xu Rui, Yang Jingming, Zhu Hong, Liu Shanshan, Jia Hongxiao

2017, 38(2):  192-196.  doi:10.3969/j.issn.1006-7795.2017.02.009

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Objective To explore the characteristics of self-processing and familiarity-processing in patients with depersonalization disorder. Methods Seventeen patients meeting the depersonalization disorder criterion of the Diagnostic and Statistical Manual of Mental Disorders, Fourth Edition (DSM-Ⅳ) and seventeen normal subjects matched for age, sex and level of education were recruited to participate in the self-face recognition test. All subjects were required to identify the identity of one certain face by pressing buttons using right hand respectively from a mixture of any two face features (self-famous, famous-stranger and self-stranger) and scrambled faces. The recognition speed to “self-face” and “other-face” were collected. The average recognition speed to the same kind of picture in different tasks was regarded as response time. Under each experimental condition, the reaction time to scrambled (control) pictures was taken as benchmark, which could be used to the reaction time ratio (corrected reaction time). Results Repeated analysis of variance (ANOVA) showed a significant main effect of group and intra group (F=20.57, P=0.001; F=3.67, P=0.031), interaction effect of group and picture was significant (F=10.75, P=0.001). Depersonalization disorder patients were significantly slower in recognizing the familiar pictures than strange pictures[(1.21±0.42) vs (1.06±0.21), P=0.001]. The reaction time ratio of both self-face[(1.12±0.30) vs (0.99±0.09)] and familiar-face[(1.21±0.42) vs (1.01±0.06)] in depersonalization disorder patients were slower than that in healthy controls (P<0.05). Pearson correlation analysis demonstrated that there was significantly positive correlations between the reacting time ratio of familiar pictures and total score of Cambridge Depersonalization Scale (CDS) (r=0.516,P=0.034)and familiarity score (r=0.498,P=0.042)in CDS. Conclusion These findings demonstrate that the reaction speed to familiar faces in depersonalization disorder patients is decreased,and further suggests that the familiarity-processing effect might be impaired in depersonalization disorder patients.

Clinical study of Jinkui Shenqi Pill to improve the fatigue of depression patients

Li Miao, Yuan Guozhen, Wang Qunsong, Zhou Zhenhe, Liu Liang

2017, 38(2):  197-201.  doi:10.3969/j.issn.1006-7795.2017.02.010

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Objective To evaluate the effectiveness of Jinkui Shenqi Pill in improving the fatigue of depression patients with kidney deficiency and liver stagnation. Methods Sixty depression patients were randomly divided into two groups:trial group of 30 cases and control group of 30 cases. The patients in the control group were treated with escitalopram oxalate,at a starting dose of 10 mg/d, at 2 weeks increased to 20 mg/d, once a day, taken in the morning at a draught. And the control group were treated with Jinkui Shenqi Pill (Zhipipian 6 g, Guizhi 12 g, Achyranthes 10 g, Plantago 15 g, Rehmannia 10 g, Alisma orientalis 10 g, cornus 12 g, Chinese yam and medicine 10g, Poria 30g, moutan 12g, one dose per day, divided to be taken twice, in the morning and evening, each time 200 mL was taken) combined with escitalopram oxalate. The treatment course was 8 weeks. The Hamilton Depression Scale (HAMD), Fatigue Self Assessment Scale (FSAS) and the Fatigue Scale-14 (FS-14) were used before and at the end of 2, 4 and 8 weeks, respectively. Results The total effective rate was 86.7% in the trial group and 60% in the control group, the difference was statistically significant (P<0.05). FS-14 score was significantly lower than that of the trial group at 2 weeks, 4 weeks and 8 weeks, the difference was significant (P<0.01). Repeated measures analysis of variance revealed a significant main effect of time (F=495.021,P=0.000), FS-14 scores of the two groups were significantly lower than those before treatment, evaluation of the interaction time groups was significant (F=40.923,P=0.000), a significant effect between groups (F=17.516, P=0.000). FSAS score was significantly lower than that of the trial group at 2 weeks, 4 weeks and 8 weeks, the difference was significant (P<0.05 or P<0.01). Repeated measures analysis of variance revealed a significant main effect of time (F=2 687.437,P=0.000), FSAS scores of the two groups were significantly lower than those before treatment, evaluation of the interaction time groups was significant (F=574.799, P=0.000), a significant effect between groups (F=8.087,P=0.006). The HAMD score of the trial group was significantly lower than that of the control group at the end of 2, 4 and 8 weeks (P<0.05 or P<0.01). Repeated measures analysis of variance revealed a significant main effect of time (F=9.374,P=0.001), the HAMD scores of the two groups were significantly lower than those before treatment, evaluation of the interaction time groups was significant (F=9.374, P=0.001), a significant interacting effect between groups (F=4.593, P=0.037). Conclusion Jinkui Shenqi pill could significantly improve the fatigue of depression patients and it is worthy of clinical application.

Progress in studies on self-face processing in patients with schizophrenia

Xu Yuanyuan, Jia Hongxiao

2017, 38(2):  202-205.  doi:10.3969/j.issn.1006-7795.2017.02.011

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Schizophrenia is a group of mental illness with unknown etiology, which has a wide range of cognitive impairment. The disease have different clinical manifestations, including perception, thinking, emotion, will obstacles and behavior inharmony. Self-disturbance is the core phenotypic marker of schizophrenia. The study of self-face recognition found that patients with schizophrenia have difficulties in identifying self-face, but no consistent results have been obtained on whether self-face processing is damaged.

Acute neuronal extracellular recording of brain in awake mice

Wang Qiushi, Lei Huimeng

2017, 38(2):  206-212.  doi:10.3969/j.issn.1006-7795.2017.02.012

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Objective Introduction to the method of acute extracellular recording of the brain of awake mice in vivo and some basic data analysis methods. Methods We applied multi-channel silicon electrode and awake mouse head-fixed system to perform acute electrophysiological recording in the awake mice. First, a head-plate adhered to the skull of the mouse, after surgery, one week's rest was given, the mouse was then mounted to the head-fixed system by screwed together the head-plate of the mouse and the head-plate holder of the head-fixed system. The limbs of the mouse could freely move in a floating ball of the head-fixed system. Three consecutive days adaptive training were performed in order to habituate the mice of the recording background, then performed the acute electrophysiological recording by use of multi-channel electrode and multi-channel electrophysiological recording system, to record the spikes and local field potentials (LFP) in neurons of awake mice, and applied Offline Sorter software to isolate the Single Unit as well as NeuroExplore software to separated the Delta, Theta, Beta and Gamma oscillations from the LFP by means of filtering. Results We successfully recorded the spikes and LFPs in neurons of awake mice, and isolated the single units as well as separated the Delta, Theta, Beta and Gamma oscillations from LFP by the analysis software. Conclusion Application of acute extracellular electrophysiological recording methods can successfully record the spikes and LFPs in neurons of awake mice.

Effects of chemical components of HSHS on the balance of inflammatory cytokines in LPS-stimulated BV2 cells

Chang Jiahui, Zhao Hui, Wang Lei, Zhang Chi, Lu Yue, Zhang Qiuxia

2017, 38(2):  213-219.  doi:10.3969/j.issn.1006-7795.2017.02.013

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Objective To investigate the influence of chlorogenic acid, cynaroside, luteolin, ginsenoside Rg1 on expression of inflammatory cytokines. Methods The experiment was divided into six groups:control, model, chlorogenic acid, cynaroside, luteolin and ginsenoside Rg1 groups. The mimic ischemia injured microglia model was induced by LPS. The cyto-activity was detected via cell count kit. The NO content was determined by Griess Reagent. Contents of TNF-α、IL-6、IL-10 and TGF-β₁ were detected by ELISA.The expression levels of p-p65 and p-IκBα were detected by Western blotting. Results Compared with those results of model group, chlorogenic acid, cynaroside, luteolin and ginsenoside Rg1 groups could significantly inhibit the release of NO, and decrease the content of TNF-α and IL-6, ginsenoside Rg1 markedly elevated the TGF-β₁ level without influencing the cell survival. Chlorogenic acid, cynaroside, luteolin and ginsenoside Rg1 groups could decrease the expression of p-p65, p-IκBα. Conclusion The findings demonstrated that chlorogenic acid, cynaroside, luteolin and ginsenoside Rg1 played regulating roles in balancing ischemia injured microglia homeostasis via promoting anti-inflammatory cytokines as well as inhibiting the inflammatory cytokines. The therapeutic roles in the inflammatory reaction of cerebral ischemia which perhaps worked through the activation of NF-κB signaling pathway.

Significance of NADPH oxidases NOX1, NOX2 and DUOX2 expression in inflammatory bowel disease

Xuan Qingxia, Dai Faliang, Chen Pan, Feng Dandan, Jin Jianjun, Gao Qiang

2017, 38(2):  220-226.  doi:10.3969/j.issn.1006-7795.2017.02.014

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Objective To investigate the expression of NOX1, NOX2 and DUOX2, the members of nicotinamide adenine dinucleotide phosphate (NADPH) oxidase family, in large intestine of patients with inflammatory bowel disease (IBD) and mouse colitis and analyze the relationship between these enzymes and IBD. Methods The samples were collected from large intestine of patients with IBD. Mouse chronic colitis model was established by using eight to ten-week-old C57BL/6 mice treated with three cycles of drinking 1.0% dextran sulfate sodium (DSS) for 7 days plus drinking water for 14 days. Mouse body weight change and hematoxylin-eosin (HE) staining of colon sections were used to evaluate the severity of inflammatory lesions. The expression of NOX1, NOX2 and DUOX2 gene and protein were detected by real-time quantitative reverse transcription polymerase chain reaction (RT-PCR) and immunohistochemistry, respectively. Results The expression of NOX1, NOX2 and DUOX2 mRNA significantly increased in human inflamed tissues compared to paired normal tissues (all P<0.01). NOX2 mRNA higher in male patients than female. Unlike human, only Nox2 mRNA increased by 2.4 times in chronic colitis mice, Nox1 and Duox2 mRNA remained unchanged. These three proteins in IBD patients increased (all P<0.01); in chronic colitis mice Nox2 and Duox2 proteins increased (both P<0.01), but Nox1 did not. Conclusion NOX1, NOX2 and DUOX2 not only play an important role in maintaining the normal physiological function, but also were associated with early onset IBD.

Study on the gastric myoelectric signal recording by implantable radiotelemetry in conscious rats with free movement

Yao Yuansheng, Zheng Lifei, Zhu Jinxia

2017, 38(2):  227-231.  doi:10.3969/j.issn.1006-7795.2017.02.015

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Objective To optimize the recording method on the gastric myoelectric activity, reduce operation trauma to animals and improve the animal survival rate after surgery. Methods The optimized recording electrodes were implanted into the serosal layer of gastric antrum. 7 days after surgery, the gastric myoelectricity signals were collected from conscious and unrestrained rats through the implantable physiological signal wireless telemetry system. The frequency, amplitude and characteristics of gastric electromyography in digestive and interdigestive phase were analyzed. Results In digestive stage, sustainable and stable gastric myoelectricity signals were recorded. The amplitude and frequency of gastric slow wave (SW) is (14.39±1.12)μV and (4.76±0.09)cpm, respectively; amplitude of gastric spike potentials (SP) is (70.76±5.31)μV and the mean number of SP on each SW is (10.38±1.22). In interdigestive phase, each cycle duration is 10-12 min, the third period of gastric migrating myoelectric/motor complex (MMC) is strongest. The amplitude and frequency of gastric SW is (14.75±0.76)μV and (4.90±0.14)cpm, respectively; amplitude of gastric SP is (72.58±3.67)μV, the mean number of SP on each SW is 12.00±1.35. In the digestion period, the use of prokinetic drug, domperidone (1mg/kg) increased the amplitude (16.87±1.05)μV to (25.77±2.16)μV (P<0.01, n=6) and frequency (4.97±0.18) cmp to (4.85±0.14)cmp (P>0.05, n=6) of SW and amplitude (72.25±3.65)μV to (104.00±7.72)μV (P<0.01, n=6) and frequency (10.50±1.12) to (14.00±0.97) (P<0.05, n=6) of SP. Conclusion Using implantable radiotelemetry, we successfully acquired rat gastric myoelectricity signals in digestive and interdigestive phase under physiological state, which provided a more accurate and effective experimental technique/method for exploring gastric myoelectric activity and then the function of gastric motility under physiologic and pathophysiologic state.

Pharmacokinetic comparison of Pyritinol in rats under normoxic and hypoxic condition

Yang Yifan, Qin Yi, Xu Weizhe, Xu Pingxiang, Xue Ming

2017, 38(2):  232-237.  doi:10.3969/j.issn.1006-7795.2017.02.016

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Objective To determine and compare the pharmacokinetic parameters of Pyritinol in rat plasma under normoxic and hypoxic conditions. Methods An effective and rapid ultra-performance liquid chromatography with tandem mass spectrometry (UPLC-MS/MS) method with positive electrospray ionization source was successfully developed and validated for quantification of Pyritinol in rat plasma. Sprague-Dawley rats were randomly divided into the hypoxia and normoxic groups. Each rat obtained a single dose of Pyritinol through intragastric administration. The plasma samples were drawn through jugular veins to measure the drug concentrations at different times. The pharmacokinetics parameters were processed via the DAS 2.0 software, and the comparison of main pharmacokinetic parameters in rats between normoxic and hypoxic groups was calculated by the SPSS software via an independent sample t test method. Results The main pharmacokinetic parameters of Pyritinol between the hypoxia and the normoxic rats were as follows:the AUC_(0-t) (88.26±9.86) ng·h·mL^-1 and (80.67±8.95)ng·h·mL^-1, MRT_(0-t) (4.14±0.32)h and (3.67±0.26)h, t_1/2 (3.72±1.82) h and (3.14±1.42)h, t_max (0.58±0.20)h and (0.67±0.26)h, C_max(30.12±2.36)ng/mL and (20.05±1.31)ng/mL, respectively. The values of C_max and MRT(o-t) for Pyritinol in hypoxic rats were statistically lower than that in normoxic rats, and other main pharmacokinetic parameters did not have significant differences. Conclusion Hypoxia affects drug peak concentration in rats, and furthermore affects the steady-state blood concentrations of drugs, which could interfere the therapeutic effect and toxic reaction.

Assessment of antagonistic effect of bone marrow mesenchymal stem cells on pulmonary fibrosis in silicosis rat model by Micro CT

An Guoliang, Li Xiaoli, Wang Yan, Lian Ximeng, Zhu Zhonghui, Guo Caixia, Wu Huihui, Tian Lin

2017, 38(2):  238-243.  doi:10.3969/j.issn.1006-7795.2017.02.017

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Objective To explore Micro CT is able to evaluate pulmonary fibrosis and reflect antagonistic effect of bone marrow mesenchymal stem cells (BMSCs) in silicosis rat model.Methods Sixty Wistar female rats were randomly divided into three groups, n=20. Silica group and BMSCs treated group rats were intratracheally given silica suspension (50 mg/mL).After silica instillation, BMSCs treated group rats were injected BMSCs by caudal vein. Other group rats were injected saline, Respectively, rat lung received computed tomography (CT) scan after silica instillation. The rat lung was fixed and stained for pathology observation after rats were sacrificed. Results Compared with the control group, at 7, 15, 30 day, silica group show that inflammatory cell infiltration, scattered silicon nodules and fused silicon nodules,respectively by pathological section HE staining.Masson staining shows that collagen fiber content was higher than those in the control group at each time point.And shadow of heighten patchy density in pulmonary hilar, punctuate with high density and high density patching shadows were observed by CT. Compared with the silica group,BMSCs treated group reveal that silicon nodules decreased pathologically and CT indicate that high density area was smaller at the 15, 30 day. Conclusion At the 15, 30 day, Micro CT is able to evaluate the degree of pulmonary fibrosis in silicosis and reflect antagonistic effect of BMSCs in silicosis rat model.

Experimental study on establishment of rat model of polycystic ovary syndrome with insulin resistance by letrozole combined with high fat diet

Cai Yun, Xu Xin

2017, 38(2):  244-248.  doi:10.3969/j.issn.1006-7795.2017.02.018

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Objective To establish a rat model of polycystic ovary syndrome with insulin resistance (PCOS-IR) by using two different types of letrozole combined with high fat diet for different periods of time to analyze their endocrine and metabolic characteristics. Methods Eighteen 6-week-old female SD rats were randomly divided into 3 groups:control, the letrozole A group and the letrozole B group. The rats in control were fed with 0.5% carboxymethylcellulose solution daily. The rats in group A received letrozole for 21 consecutive days and fed with high-fat diet. Group B received letrozole for 30 days, feeding with high fat diet. The levels of follicle stimulating hormone (FSH), luteinizing hormone (LH), testosterone (T), triglyceride (TG) and fasting insulin (FINS) were measured and the changes of body weight and ovarian histology were observed. LH/FSH ratio and insulin resistance index (HOMA-IR) were calculated. Results Compared with the control, the body weight of letrozole A group increased significantly(P<0.05). The sex hormones and metabolic indexes were higher than that in the control (P<0.05), but there was no statistically significant difference (P>0.05).The weight of rats in group B was significantly higher than that in the control (P<0.05). The levels of T,LH/FSH,TG, FPG, FINS and HOMA-IR were significantly increased (P<0.01). Conclusion Letrozole combined with high fat diet for 30 days can successfully induce a rat model of polycystic ovary syndrome with insulin resistance.

Dendritic cells on mice psoriasis-like lesions induced by imiquimod and the effects of Liangxue Jiedu decoction

Wang Mingxing, Wang Yan, Zhao Jingxia, Di Tingting, Ruan Zhitong, Meng Yujiao, Xie Xiangjiang, Zhang Lu, Lin Yan, Wang Ning, Li Ping

2017, 38(2):  249-259.  doi:10.3969/j.issn.1006-7795.2017.02.019

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Objective To observe the effects of Liangxue Jiedu decoction(LXJD) on the abnormal activation of dendritic cells on mice psoriasis-like lesions induced by imiquimod. Methods Thirty-six BALB/c mice were randomly divided into six groups:control group, model group, Liangxue Jiedu groups with high(LXJD-H),medium(LXJD-M) or low(LXJD-L) doses, and Methotrexate (MTX) group. The lesions were evaluated according to the psoriasis area and severity index(PASI). The histology and epidermal thicknesses were observed under light microscope.The expression of Ki-67 protein was detected by influorescent antibody technique. Meanwhile, the positive expression of CD3, CD11c was counted by immunohistochemical staining.The expression of dendritic cells in mouse spleen cells and the content of IL-23, IL-12p40, IL-1β in DC2.4 by flow cytometry. The IL-23 mRNA, TLR7, TLR8 were detected by PCR. The expression of TLR7 protein was detected by Western blotting. Results Compared with model group, the cutaneous symptoms in LXJD capsules groups were alleviated, with PASI scores decreased, epidermal parakeratosis and epidermal over-proliferation, the numbers of dermal T lymphocytes, dendritic cells reduced significantly.Compared with model group, the number of Ki67, CD3 and CD11c⁺ in LXJD group was reduced obviously. Meanwhile the expression of IL-23, TLR7, TLR8 and IL-12p40 were all decreased compared with model group. Then in the cell experiment the secretion of cytokines IL-23 and IL-1β were all decreased. Conclusion Liangxue Jiedu Decoction could improve IMQ-induced mouse psoriasis-like lesions and ameliorate the infiltration of T cells and DCs in psoriasis-like lesions, While inhibiting the activation of DCs.

Safety evaluation during deep-brain magnetic stimulation (DMS)treating motor symptoms in Parkinsonian rats

Liu Junhua, Wang Yong, Wang Yanyan, Wang Xiaomin

2017, 38(2):  260-267.  doi:10.3969/j.issn.1006-7795.2017.02.020

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Objective To explore the security via DMS to treat motor symptoms in hemi-parkinsonian rats.Methods Rats were injected with 6-hydroxydopamine (6-OHDA) into right striatum to establish unilateral parkinsonian model. Sham group received the same volume of normal saline by the same procedure. After screened via apomorphine, the successful parkinsonian rats were randomly grouped into model, δ rhythm DMS treatment (DMS-δ) and γ rhythm DMS treatment (DMS-γ). Among each DMS treatment group, 40 minutes trains of DMS were administered daily for 4 weeks. Cylinder tests were conducted to detect motor outcomes. Body weight determination, peripheral blood lymphocytes classification & counting, HE staining were executed to evaluate the security of DMS. Results 1The lesioned side forelimb usage ratio in Sham and DMS-γ groups were significantly higher than that in Model group. 2There was no significant difference of body weight between each group, indicating that DMS had no significant effects on body weight. 3There was no significant difference of the number and percentage of total lymphocytes & T cells between each group, while the natural killer (NK) cells number and percentage in DMS-δ group were significantly higher than that in Model group. 4Results of HE staining for major organs showed no significant histopathological change. Conclusion DMS-γ treatment alleviated bilateral limb asymmetry in PD rats. Meanwhile, DMS treatment showed no significant side effects, indicating that DMS showed satisfactory security in the treatment of motor symptoms in PD rats.

Effect of epalrestat on liver injury in type 2 diabetic rats

Zhao Yuerong, Hou Biyu, Liu Chenge, Wang Xiaobo, Du Guanhua, Zhang Li, Guan Shuyu

2017, 38(2):  268-276.  doi:10.3969/j.issn.1006-7795.2017.02.021

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Objective This study investigated the effect of epalrestat on liver injury in rats with type 2 diabetic mellitus (T2DM). Methods SD rats were fed with high-fat and high-sucrose diet for four weeks, then 45 mg·kg^-1 Streptozotocin (STZ) was injected intraperitoneally to make the animal model of type 2 diabetes. Diabetic rats were divided into two groups as the model group and the epalrestat group (100 mg·kg^-1), another 12 normal SD rats were served as the control group. The diabetic rats were treated with or without epalrestat by gavaging for 8 weeks. Body weight, food and water intake, blood glucose level, blood lipids were measured; liver function, liver pathological changes and oxidative stress were examined. Immunohistochemistry of α-smooth muscle actin (α-SMA)and transforming growth factor-β1 (TGF-β1) were conducted. Results Compared with the model group, epalrestat could reduce the concentration of fasting plasma glucose and blood lipids. The index for liver condition and oxidative stress were down regulated. Pathological examination showed that lesions of hepatic inflammation and fibrosis were significantly alleviated. The expressions of α-SMA and TGF-β1 were markedly reduced in liver tissues of type 2 diabetic rats with the treatment of epalrestat. Conclusion Epalrestat could alleviate liver inflammation and hepatic fibrosis of rats with T2DM via anti-oxidative stress and inhibiting the activation of hepatic stellate cell.

Signaling of bone marrow mesenchymal stem cell differentiation to myofibroblasts mediated by S1PR1/3

Yang Yuanru, Zhou Xuan, Zhang Ruochen, Li Liying, Yang Le

2017, 38(2):  277-281.  doi:10.3969/j.issn.1006-7795.2017.02.022

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Objective Aim of this study is to explore the role of RhoA in transforming growth factor β1 (TGFβ1)-induced bone marrow mesenchymal stem cell (BMSC) differentiation to myofibroblast (MF) mediated by sphingosine 1-phosphate receptor 1/3 (S1PR1/3). Methods Serum-starved primary cultured mouse BMSCs were stimulated by a series dose of TGFβ1. Expression of α-smooth muscle actin (αSMA), procollagen α1(Ⅰ)[Col α1(Ⅰ)] and procollagen α1(Ⅲ)[Col α1(Ⅲ)] was measured by qRT-PCR. Activated RhoA induced by TGFβ1 in BMSCs was determined by Pull-down assay. Results TGFβ1 induced an up-regulation of αSMA, Col α1(Ⅰ) and Col α1(Ⅲ) mRNA levels in BMSCs. TGFβ1 triggered the activation of RhoA, which can be blocked by S1PR1/3 antagonists. RhoA inhibitor C3 Transferase reversed the up-regulation of αSMA, Col α1(Ⅰ) and Col α1(Ⅲ) mRNA expression induced by TGFβ1. Conclusion RhoA is involved in the differentiation of BMSC to MF induced by TGFβ1.

Effects of Jieduan Niwan Formula on the c-Jun,p-JNK of liver tissue in Acute-on-Chronic liver failure rats

Mu Lingyun, Li Jinxia, Zhang Qiuyun, Chen Yu, Gao Lianyin, Du Yuqiong

2017, 38(2):  282-288.  doi:10.3969/j.issn.1006-7795.2017.02.023

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Objective To observe the effect of the Jieduan-Niwan Formula (JDNW)on the serum tumor necrosis factor alpha(TNF-α)content,the expression of p-JNK and transcription factor c-Jun in acute-on-chronic liver failure(ACLF)rats,to discuss part of the action mechanism of intervention of in ACLF. Methods SPF 70 male Wistar rats were randomly divided into normal control group,model group,JDNW group and JNK inhibitor SP600125 group,to inject pig serum to induce rat liver fibrosis model,and then acute attack combined with D-GalN/LPS,ACLF model was established. Rats in JDNW group were orally given JDNW formula for 3 days before acute attack.Rats in SP600125 group giving intraperitoneal injection half an hour before acute attack.Rats were sacrificed, respectively at 4,8 and 12 h after model established.The expression of TNF-α,c-Jun,p-JNK were detected by enzyme-linked immune sorbent assay(ELISA),western blot method and immunohistochemical analysis, respectively. Results Compared with normal group,the expression of TNF-α,p-JNK,c-Jun of model group were gradually increased;compared with the model group,the expression of TNF-α,p-JNK,c-Jun of JDNW and SP600125 group in 4h and 8h was less(P<0.05),and the expression of 12h increased; the different expression of SP600125 group was in accordance with the JDNW group. Conclusion The prescription of JDNWcan reduce the damage of rat liver cell ACLF in a certain extent,its mechanism may be through cell apoptosis mediated by JNK signaling pathway,thereby protecting liver cells,inhibiting liver failure.

Effects of glycated albumin on inducing injury and inflammation in human proximal tubular epithelial cells

Duan Nan, Liu Yi, Li Haixia[KG-*2], Jiao Lili

2017, 38(2):  289-294.  doi:10.3969/j.issn.1006-7795.2017.02.024

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Objective To investigate the effects of glycated albumin (GA) on the expression of kidney injury molecule-1 (KIM-1), neutrophil gelatinase-associated lipocalin (NGAL) and inflammatory cytokines in human proximal tubular epithelial cells (HK-2 cells). Methods The HK-2 cells were treated with the different concentrations of GA and albumin (Alb) for 12h, 24h and 48h. The mRNA expressions of KIM-1 and NGAL were detected by real-time PCR. The release of KIM-1 and NGAL in the supernatants were detected by ELISA. The concentrations of VEGF, TNF-α, IL-8 and sICAM-1 were detected by cytometric beads array method. Results Compared with control and Alb groups, the mRNA levels of KIM-1 and NGAL in HK-2 cells were significantly up-regulated at 12 h, 24 h and 48 h after GA treatment (P<0.05); the protein levels of KIM-1 and NGAL released in supernatants of GA-treated cells were significantly higher than those in control group and Alb groups at the same time points (P<0.05); GA groups also had significantly higher levels of sICAM-1, VEGF and IL-8 than control group at each time point (P<0.05). Conclusion GA can up-regulate the expression and release of KIM-1 and NGAL and promote the secretion of inflammatory cytokines which could cause damage to renal tubules, suggesting that GA may reflect the diabetic renal involvement.

Current status of clinical management for small-bowel diseases in Beijing tertiary hospitals

Qi Shirui, Chen Jun, Pan Fei, Luan Zhe, Sun Gang

2017, 38(2):  295-298.  doi:10.3969/j.issn.1006-7795.2017.02.025

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Objective To investigate the current diagnosis and treatment status of small-bowel diseases and clinical application of small-bowel endoscopy in Beijing tertiary-level hospitals. Methods The internet-based questionnaire was used to investigate the application of capsule endoscopy (CE) and balloon assisted enteroscopy (BAE), proficiency in current guidelines and continuing medical education (CME) activities, as well as management strategy for obscure gastrointestinal bleeding (OGIB). Results Twenty nine tertiary-level hospitals in Beijing were investigated. 24 (82.8%) hospitals equipped with CE and 12 (41.4%) with BAE. The median application time was 5-6 years. Median cases received CE per hospital one year was 50-100 with a failure rate of 1%-3%. While median cases received BAE per hospital one year was less than 50 with a failure rate of 3%-5%. The incidence of severe complications of CE and BAE was less than 1% and overall diagnosis rate was 50%-60%. The positive rate of CE of each hospital was positive correlation with received CE cases per year(r=0.542,P=0.005). Only half of the respondents mastered the guideline of CE and 20.7% never attended CME program. Respondents engaged in small-bowel endoscopy working had different diagnosis strategies for first diagnosed negative OGIB patients with those not engaged in small-bowel endoscopy working(χ²=3.898,P=0.048). Conclusion More hospitals are equipped with CE than BAE in Beijing tertiary-level hospitals. Both CE and BAE are safe for clinic use with similar diagnostic yield of 50%-60%. Physicians have insufficient knowledge of guideline and standard process of CE and more continued medical education (CME) should be recommended in the future. The procedure of diagnosis and treatment for OGIB needs to be further standardized.

Value of instantaneous elastic measurement on non invasive diagnosis of early hepatitis B -related liver cirrhosis

Wang Tiezheng, Wu Jushan, Meng Fankun, Zang Yunjin, Lin Dongdong, Li Jian, Li Ning

2017, 38(2):  299-303.  doi:10.3969/j.issn.1006-7795.2017.02.026

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Objective To study the relationship among the transient elasticity measurement and the fibrosis staging and free portal pressure, explore the diagnostic value and influence factors of transient elastography in early hepatitis B -related liver cirrhosis.Methods Sixty patients who were undertaken splenectomy and devascularization and liver biopsy surgery from January 2014 to May 2016 in Youan Hospital affiliated to Capital Medical University were involved. We detected liver stiffness measurement (LSM)with transient elastographypreoperatively, measured free portal pressure(FPP)and took Wedge biopsy of liver intraoperatively, analyzed in conjunction with clinical and pathologic data. Results LSM was (17.6±13.9) kPa, presplenectomy free portal pressure was (34.99±6.52)cmH₂O (1 cmH₂O=0.098 kPa), postsplenectomy free portal pressure was (25.52±4.50)cmH₂O, postdevascularization free portal pressure was (26.28±4.36)cmH₂O. Data of three groups had a significant difference (P<0.05). According topathological diagnosis,S0 3 cases, S1 7cases, S2 8cases, S3 6cases, S4 36 cases. G1 45 cases, G1-G2 9cases, G2 6 cases. The area under the curve (AUC) for diagnosis of cirrhosis was 0.876, cut-off value 7.7 kPa,the sensitivity and specificity were 100% and 66.67% by transient elasticity measurement. LSM was positively correlated with the stage of liver fibrosis (r=0.684,P<0.05). Multiple linear regression revealed that AFP was an independent factor for LSM.Conclusion Measurement of transient elastography had important reference value with early non-invasive diagnostic in decompensated cirrhosis.

Diffusion kurtosis imaging study: the effects of ApoEε4 epsilon4 alleles on brain microstructure in healthy elders

Zhao Yanling, Wang Jun, Wang Xiaochun, Zhang Hui, Hao Xiaoyong, Li Dan

2017, 38(2):  304-307.  doi:10.3969/j.issn.1006-7795.2017.02.027

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Objective To compare the difference of the brain microstructure between apolipoprotein Eε4 (ApoEε4) allele carriers and noncarriers of normal elderly people with normal cognitive state and to explore the influence of ApoEε4 alleles on its brain microstructure by using diffusion kurtosis imaging (DKI) magnetic resonance technology. Methods The ApoEε4 alleles was detected in oral mucosa of 18 normal elders; All subjects underwent routine magnetic resonance imaging(MRI) scanning and DKI scanning. Mean kurtosis(MK) and fractional anisotraphy(FA) values were measured in bilateral frontal lobe, parietal lobe, temporal lobe, posterior cingulate cortex, splenium of corpus callosum and hippocampe. The two-sample t-test was used to compare the parameters between the ApoEε4 allele carriers and noncarriers. Results MK value in the temporal lobe and hippocampus of the ApoEε4 allele carriers was significantly lower than in that of noncarriers (P<0.05). FA value in the hippocampus of the ApoEε4 allele carriers was significantly lower than in that of noncarriers (P<0.05). Conclusion Microstructural changes of the brain of ApoEε4 alleles carriers in normal elderly persons with normal cognitive state are more obvious. DKI could detect the influence of ApoEε4 allele on the microstructural changes of the brain in the eraly time.

Clinical study on circulating VCAM-1 to evaluate the metastasis risk of mastoscopic axillary lymph node dissection

Luo Chengyu, Fan Kemin, Ge Shasha, Cui Zhimiao

2017, 38(2):  308-312.  doi:10.3969/j.issn.1006-7795.2017.02.028

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Objective To elucidate the change after mastoscopic axillary lymph node dissection (MALND) and conventional axillary lymph node dissection (CALND) and further prove the safety of MALND.Methods 104 breast cancer patients (January 1st, 2014 to July 31 st, 2015) from Breast Minimally Invasive Center, Fuxing Hospital,Capital Medical University who were undergone ALND were randomly divided into two groups:CALND(n=52) and MALND(n=52). The peripheral blood samples were respectively collected in pre-operation and 1,3,5 days after operation. The circulating levels of vascular cell adhesion molecular-1 (VCAM-1) in blood were analyzed by ELISA. Results Circulating VCAM-1 levels of CALND group on postoperative 1 d(51.74±1.37)ng/mL,3 d(52.64±1.24)ng/mL,5 d(52.22±1.29)ng/mL decreased significantly than that of pre-operation (56.56±1.24)ng/mL. And the figures of MALND group turn to be(53.24±1.21)ng/mL,(52.07±1.34)ng/mL,(48.99±1.27)ng/mL,(58.66±0.95) ng/mL. The VCAM-1 levels of MALND group is lower than that of CALND, but there was no statistical difference between the two groups (P>0.05). Conclusion The change of circulating VCAM-1of patients after MALND is same to that of CALND. There is no evidence that MALND increases the risk of blood dissemination of tumor.

Effects of mild sedation on neurologic function in patients with supratentorial mass lesion and the associated factors in head magnetic resonance imaging

Lin Nan, Zhou Jianxin, Han Ruquan

2017, 38(2):  313-319.  doi:10.3969/j.issn.1006-7795.2017.02.029

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Objective Sedation may induce or exacerbate neurologic function in some patients with supratentorial mass lesion. This trial aimed to investigate the risk factor of sedative associated neurologic deficits in that population through analyzing head magnetic resonance imaging (MRI). Methods The study was a prospective, randomized, single-blind, controlled trial. Patients were randomly assigned to one of the four study groups, including 'Propofol group', 'Midazolam group', 'Fentanyl group' or 'Dexmedetomidine group'. In each group, patients were titrated to Observer's Assessment of Alertness and Sedation (OAA/S) score 4 with a ladder-like administration of the assigned drug to target this same sedation level. National Institutes of Health Stroke Scale (NIHSS) was applied to evaluate before and after the sedation. Patients' magnetic resonance imaging (MRI) findings were collected after the evaluation. Results This study showed that mild sedation unmasked or exacerbated neurologic deficits in patients with supratentorial brain tumors. Patients with brain tumors in the primary motor area(P=0.000),primary sensory area(P=0.004), basil ganglion(P=0.031), with brain midline shift(P=0.000), brain ventricular compression or expansion(P=0.000) as well as present peritumor edema(P<0.001)had a higher probability of sedative related neurologic deficits. Conclusion The neurologic function altering is more sensitive to sedation if MRI displays a primary motor area mass lesion and brain midline shift. However the potential mechanism of sedative related neurologic deficits is still in need of further investigation.

Clinical observation on surface electromyography value of pelvic muscle under biofeedback treatment in postpartum stress urinary incontinence

Wang Hong, Xue Ziyi, Li Mengmeng, Liu Jun, Zhang Zhenyu

2017, 38(2):  320-324.  doi:10.3969/j.issn.1006-7795.2017.02.030

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Objective To access the effect of biofeedback treatment in postpartum stress urinary incontinence under biofeedback treatment and the surfaceelectromyography value of pelvic muscle. Methods Sixty two mild postpartum stress urinary incontinence(SUI) patients were divided into 2 groups under biofeedback treatment with SOKO9003 therapy instrument. The clinical effects between 2 groups were compared and the surface electromyography value of pelvic muscle,Modified Oxford Scale (MOS) were recorded and compared before and after treatment. Results The effective rate was 87.1(54/62)% after 5-week treatment and 77.4(48/62)%after 6-month treatment. It is increased after treatment in both groups(P<0.05). The effect is better in vaginal delivery (VD) group than in caesarean section (CS) group(P<0.05). After biofeedback treatment the difference of surface electromyography value of pelvic muscle were significantly increased in 2 groups(P<0.05), while the differences were statistically no significant in the treatment times(P>0.05). Conclusion The treatment effect was obviously both in 2 groups after biofeedback. Pelvic floor recovered better in VD group. Vaginal delivery is recoverable damage in pelvic floor.

Clinical analysis of urinary complications of ¹²⁵I seeds implant in patients of low-risk prostate cancer

Zhang Jiwei, Yan Yifu, Xia Ming

2017, 38(2):  325-328.  doi:10.3969/j.issn.1006-7795.2017.02.031

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Objective To analyze the side effects and complication of low-risk prostate cancer after ¹²⁵I seeds prostate implant. Methods A total of 30 prostate cancer patients with age ranging from 65 to 85 years[average age of (78.1±5.30) years] were recruited. All patients were treated between January 2007 and June 2015. The prostate specific antigen (PSA) level of these patients was 4.15-9.8 ng/mL[average (8.25±1.04) ng/mL], and the Gleasons Score was 5-7. All patients were clinically staged as T_1c-T_2aN₀M₀; 30 cases with prostate volume of 38-55 mL, an average of (49.1±3.92) mL. Under continual epidural anesthesia, these patients underwent trans-rectal ultrasound. Pictures were transmitted to the computer system to make plan. According to the plan, patient received ¹²⁵I seed brachytherapy under the guidance of the trans-rectal ultrasound. Results All 30 cases were implanted seeds of 45-70 (average seeds of 52.6±6.77). The follow-up period was 12-86 months, with average of (40.4±7.01) months. The urinary side effects of gradeⅠ, Ⅱ, Ⅲ, and Ⅳ were 66.67% and 20% and 6.67% and 0 one month after seeds implant. By conservative treatment, the urinary side effects of gradeⅠand Ⅱwere 10% and 3.33% 12 months after seeds implant. No grade Ⅲ and Ⅳ side effects were found. Symptom remission rate was 85.71%. Conclusion Urinary side effects are common after prostate brachytherapy but are mostly self-limiting in the majority of patients, such side effects resolve within a year after the procedure. Serious complications of grade Ⅳ are rare. Intraoperative accurate localization and making plan and implantation are the key to the prevention of serious complications.