Acute neuronal extracellular recording of brain in awake mice

doi:10.3969/j.issn.1006-7795.2017.02.012

Abstract

Abstract: Objective Introduction to the method of acute extracellular recording of the brain of awake mice in vivo and some basic data analysis methods. Methods We applied multi-channel silicon electrode and awake mouse head-fixed system to perform acute electrophysiological recording in the awake mice. First, a head-plate adhered to the skull of the mouse, after surgery, one week's rest was given, the mouse was then mounted to the head-fixed system by screwed together the head-plate of the mouse and the head-plate holder of the head-fixed system. The limbs of the mouse could freely move in a floating ball of the head-fixed system. Three consecutive days adaptive training were performed in order to habituate the mice of the recording background, then performed the acute electrophysiological recording by use of multi-channel electrode and multi-channel electrophysiological recording system, to record the spikes and local field potentials (LFP) in neurons of awake mice, and applied Offline Sorter software to isolate the Single Unit as well as NeuroExplore software to separated the Delta, Theta, Beta and Gamma oscillations from the LFP by means of filtering. Results We successfully recorded the spikes and LFPs in neurons of awake mice, and isolated the single units as well as separated the Delta, Theta, Beta and Gamma oscillations from LFP by the analysis software. Conclusion Application of acute extracellular electrophysiological recording methods can successfully record the spikes and LFPs in neurons of awake mice.

Key words: awake, acute, in vivo, extracellular, electrophysiology

CLC Number:

R338

Wang Qiushi, Lei Huimeng. Acute neuronal extracellular recording of brain in awake mice[J]. Journal of Capital Medical University, 2017, 38(2): 206-212.

0
/ / Recommend

Add to citation manager EndNote|Reference Manager|ProCite|BibTeX|RefWorks

URL: https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/10.3969/j.issn.1006-7795.2017.02.012

https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/Y2017/V38/I2/206

References

[1] Buzsáki G. Large-scale recording of neuronal ensembles[J]. Nat Neurosci, 2004, 7(5):446-451.
[2] Gold C, Henze D A, Koch C, et al. On the origin of the extracellular action potential waveform: a modeling study[J]. J Neurophysiol, 2006, 95(5):3113-3128.
[3] Henze D A, Borhegyi Z, Csicsvari J, et al. Intracellular features predicted by extracellular recordings in the hippocampus in vivo[J]. J Neurophysiol, 2000, 84(1):390-400.
[4] Lyons-Warren A M, Kohashi T, Mennerick S, et al. Retrograde fluorescent labeling allows for targeted extracellular single-unit recording from identified neurons in vivo[J]. J Vis Exp, 2013 (76).doi: 10.3791/3921.
[5] Paxinos G, Franklin K B J. The mouse brain in stereotaxic coordinates(second edition )[M]. San Diego: Acadimic Press, 1997.
[6] Sofroniew N J, Cohen J D, Lee A K, et al. Natural whisker-guided behavior by head-fixed mice in tactile virtual reality[J]. J Neurosci, 2014,34(29):9537-9550.
[7] Kislin M, Mugantseva E, Molotkov D, et al. Flat-floored air-lifted platform: a new method for combining behavior with microscopy or electrophysiology on awake freely moving rodents[J]. J Vis Exp, 2014(88):e51869.
[8] DiCarlo J J, Lane J W, Hsiao S S, et al. Marking microelectrode penetrations with fluorescent dyes[J]. J Neurosci Methods, 1996,64(1):75-81.
[9] 寿天德.神经生物学[M].2版. 北京:高等教育出版社, 2006: 23-26.
[10] White J J, Lin T, Brown A M, et al. An optimized surgical approach for obtaining stable extracellular single-unit recordings from the cerebellum of head-fixed behaving mice[J]. J Neurosci Methods, 2016,262:21-31.
[11] Schmitzer-Torbert N, Jackson J, Henze D, et al. Quantitative measures of cluster quality for use in extracellular recordings[J]. Neuroscience, 2005,131(1):1-11.
[12] Ness T V, Remme M W, Einevoll G T.Active subthreshold dendritic conductances shape the local field potential[J]. J Physiol, 2016, 594(13):3809-3825.
[13] Berens P, Keliris G A, Ecker A S, et al. Feature selectivity of the gamma-band of the local field potential in primate primary visual cortex[J]. Front Neurosci, 2008, 2(2): 199-207.
[14] Thompson J A, Lanctin D, Ince N F, et al. Clinical implications of local field potentials for understanding and treating movement disorders[J]. Stereotact Funct Neurosurg, 2014, 92(4):251-263.
[15] Airaksinen A M, Niskanen J P, Chamberlain R, et al. Simultaneous fMRI and local field potential measurements during epileptic seizures in medetomidine-sedated rats using raser pulse sequence[J]. Magn Reson Med, 2010, 64(4):1191-1199.
[16] Jessberger J, Zhong W, Branka c ∨ k J, et al. Olfactory bulb field potentials and respiration in sleep-wake states of mice[J]. Neural Plast, 2016, 2016:4570831.
[17] Martin C, Ravel N. Beta and gamma oscillatory activities associated with olfactory memory tasks: different rhythms for different functional networks?[J]. Front Behav Neurosci, 2014, 8:218.
[18] Bender F, Gorbati M, Cadavieco M C, et al. Theta oscillations regulate the speed of locomotion via a hippocampus to lateral septum pathway[J]. Nat Commun, 2015, 6:8521.
[19] Vandecasteele M, Varga V, Berényi A, et al. Optogenetic activation of septal cholinergic neurons suppresses sharp wave ripples and enhances theta oscillations in the hippocampus[J]. Proc Natl Acad Sci U S A, 2014, 111(37):13535-13540.