Signaling of bone marrow mesenchymal stem cell differentiation to myofibroblasts mediated by S1PR1/3

doi:10.3969/j.issn.1006-7795.2017.02.022

Abstract

Abstract: Objective Aim of this study is to explore the role of RhoA in transforming growth factor β1 (TGFβ1)-induced bone marrow mesenchymal stem cell (BMSC) differentiation to myofibroblast (MF) mediated by sphingosine 1-phosphate receptor 1/3 (S1PR1/3). Methods Serum-starved primary cultured mouse BMSCs were stimulated by a series dose of TGFβ1. Expression of α-smooth muscle actin (αSMA), procollagen α1(Ⅰ)[Col α1(Ⅰ)] and procollagen α1(Ⅲ)[Col α1(Ⅲ)] was measured by qRT-PCR. Activated RhoA induced by TGFβ1 in BMSCs was determined by Pull-down assay. Results TGFβ1 induced an up-regulation of αSMA, Col α1(Ⅰ) and Col α1(Ⅲ) mRNA levels in BMSCs. TGFβ1 triggered the activation of RhoA, which can be blocked by S1PR1/3 antagonists. RhoA inhibitor C3 Transferase reversed the up-regulation of αSMA, Col α1(Ⅰ) and Col α1(Ⅲ) mRNA expression induced by TGFβ1. Conclusion RhoA is involved in the differentiation of BMSC to MF induced by TGFβ1.

Key words: mouse, bone marrow mesenchymal stem cell, sphingosine 1-phosphate receptor, RhoA

CLC Number:

Yang Yuanru, Zhou Xuan, Zhang Ruochen, Li Liying, Yang Le. Signaling of bone marrow mesenchymal stem cell differentiation to myofibroblasts mediated by S1PR1/3[J]. Journal of Capital Medical University, 2017, 38(2): 277-281.

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URL: https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/10.3969/j.issn.1006-7795.2017.02.022

https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/Y2017/V38/I2/277

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