Assessment of antagonistic effect of bone marrow mesenchymal stem cells on pulmonary fibrosis in silicosis rat model by Micro CT

doi:10.3969/j.issn.1006-7795.2017.02.017

Abstract

Abstract: Objective To explore Micro CT is able to evaluate pulmonary fibrosis and reflect antagonistic effect of bone marrow mesenchymal stem cells (BMSCs) in silicosis rat model.Methods Sixty Wistar female rats were randomly divided into three groups, n=20. Silica group and BMSCs treated group rats were intratracheally given silica suspension (50 mg/mL).After silica instillation, BMSCs treated group rats were injected BMSCs by caudal vein. Other group rats were injected saline, Respectively, rat lung received computed tomography (CT) scan after silica instillation. The rat lung was fixed and stained for pathology observation after rats were sacrificed. Results Compared with the control group, at 7, 15, 30 day, silica group show that inflammatory cell infiltration, scattered silicon nodules and fused silicon nodules,respectively by pathological section HE staining.Masson staining shows that collagen fiber content was higher than those in the control group at each time point.And shadow of heighten patchy density in pulmonary hilar, punctuate with high density and high density patching shadows were observed by CT. Compared with the silica group,BMSCs treated group reveal that silicon nodules decreased pathologically and CT indicate that high density area was smaller at the 15, 30 day. Conclusion At the 15, 30 day, Micro CT is able to evaluate the degree of pulmonary fibrosis in silicosis and reflect antagonistic effect of BMSCs in silicosis rat model.

Key words: Micro CT, silicosis, bone marrow mesenchymal stem cells

CLC Number:

An Guoliang, Li Xiaoli, Wang Yan, Lian Ximeng, Zhu Zhonghui, Guo Caixia, Wu Huihui, Tian Lin. Assessment of antagonistic effect of bone marrow mesenchymal stem cells on pulmonary fibrosis in silicosis rat model by Micro CT[J]. Journal of Capital Medical University, 2017, 38(2): 238-243.

0
/ / Recommend

Add to citation manager EndNote|Reference Manager|ProCite|BibTeX|RefWorks

URL: https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/10.3969/j.issn.1006-7795.2017.02.017

https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/Y2017/V38/I2/238

References

[1] Leung C C, Yu I T, Chen W. Silicosis [J]. Lancet, 2012, 379(9830):2008-2018.
[2] Wang X, Zhao W, Wang J, et al. Bone marrow stromal cells inhibit the activation of liver cirrhotic fat-storing cells via adrenomedullin secretion [J]. Dig Dis Sci, 2015, 60(5):1325-1334.
[3] Ali G, Masoud M S. Bone marrow cells ameliorate liver fibrosis and express albumin after transplantation in CCl₄-induced fibrotic liver [J]. Saudi J Gastroenterol, 2012, 18(4):263-267.
[4] Hong L, Dai C. Effects of bone marrow mesenchymal stem cells on plasminogen activator inhibitor-1 and renal fibrosis in rats with diabetic nephropathy [J]. Arch Med Res, 2016, 47(2):71-77.
[5] Ni S, Wang D, Qiu X, et al. Bone marrow mesenchymal stem cells protect against bleomycin-induced pulmonary fibrosis in rat by activating Nrf2 signaling [J]. Int J Clin Exp Pathol, 2015, 8(7):7752-7761.
[6] Greenberg M I, Waksman J, Curtis J. Silicosis: a review [J]. Dis Mon, 2007, 53(53):394-416.
[7] 吴天文. CT在矽肺诊断中与X线的优势对比[J]. 中国医药指南, 2011, 9(14):24-25.
[8] 袁功玲, 李敏, 沈苏军,等. 矽肺病人36例的CT影像学分析[J]. 职业与健康, 2013, 29(13):1587-1589.
[9] 武粟, 唐洪渠,孔亮.煤工尘肺大阴影的CT表现及临床意义[J]. 中国煤炭工业医学杂志,2016 19(4):555-557.
[10] Zhao M M, Cui J Z, Cui Y, et al. Therapeutic effect of exogenous bone marrow derived mesenchymal stem cell transplantation on silicosis via paracrine mechanisms in rats [J]. Mol Med Rep, 2013, 8(3):741-746.
[11] Huang K, Kang X, Wang X, et al. Conversion of bone marrow mesenchymal stem cells into type Ⅱ alveolar epithelial cells reduces pulmonary fibrosis by decreasing oxidative stress in rats [J]. Mol Med Re, 2015, 11(3):1685-1692.
[12] 郝新民. 应用Meta分析CT与X线在尘肺病诊断的优势对比[D].杭州: 浙江大学, 2013.