Comparison of murine decidual natural killer cells shown by Periodic Acid Schiff's and Dolichos Bifows Agglutinin reactions

doi:10.3969/j.issn.1006-7795.2013.01.022

Journal of Capital Medical University ›› 2013, Vol. 34 ›› Issue (1): 115-119.doi: 10.3969/j.issn.1006-7795.2013.01.022

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Comparison of murine decidual natural killer cells shown by Periodic Acid Schiff's and Dolichos Bifows Agglutinin reactions

LU Xin¹, XU Qing², WENG Jing³

1. Morphological Laboratory, Center for Basic Medical Experimental Teaching, School of Basic Medical Science, Capital Medical University, Beijing 100069, China;
2. Medical Experiments and Testing Center, Capital Medical University, Beijing 100069, China;
3. Department of Histology and Embryology, School of Basic Medical Science, Capital Medical University, Beijing 100069, China

Received:2012-07-04 Online:2013-02-21 Published:2013-02-25
Supported by:
This study was supported by Scientific Research Key Program of Beijing Municipal Commission of Education (KM200910025005).

Abstract

Abstract:

To identify the difference of mouse decidual natural killer (dNK) cells reacting to Periodic Acid Schiff's (PAS) and Dolichos Biflorus agglutinin (DBA). Methods The dNK cells of mice uteri Gd 3 to Gd 19 (using the copulation plug as Gd1) were shown on continuous paraffin sections by PAS, DBA, PAS/DBA double reactions protocol. Results Typical dNK cells contained numerous PAS positive reactive cytoplasmic granules. The cytoplasmic membrane, nuclear membrane and granule membrane of dNK were positive reaction to DBA due to their specific binding. The immature dNK cells in the uterus of early pregnancy (on Gd 3 to Gd 6) were negative to PAS because of their absence of granules. Majority of dNK cells could be labeled at the same time with DBA plus PAS double staining. Conclusion DBA can be used as a new marker to show dNK due to its specific binding to membranes. DBA has advantage especially in early pregnancy (such as on particles Gd 3) because PAS positive granules have not yet appeared in immature dNK cells.

Key words: decidual natural killer cells, Periodic Acid-Schiff reaction, Dolichos Bifows Agglutinin, mouse

CLC Number:

R711

LU Xin, XU Qing, WENG Jing. Comparison of murine decidual natural killer cells shown by Periodic Acid Schiff's and Dolichos Bifows Agglutinin reactions[J]. Journal of Capital Medical University, 2013, 34(1): 115-119.

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Comparison of murine decidual natural killer cells shown by Periodic Acid Schiff's and Dolichos Bifows Agglutinin reactions

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