Differential expressions of α-synuclein and glucocerebrosidase in different brain regions of cynomolgus monkeys

doi:10.3969/j.issn.1006-7795.2013.01.021

Abstract

Abstract:

Objective To investigate the expressions of α-synuclein (α-Syn) and glucocerebrosidase (GBA) in different brain regions of cynomolgus monkeys. Methods Western blotting and immunohistochemical methods were applied to measure the levels of α-Syn and GBA in striatum and cerebellum of cynomolgus monkeys. Enzyme activity assay was used to determine GBA activity. Double immunofluorescent labeling was used to examine the co-localization of α-Syn and GBA. Results α-Syn and GBA were colocalized in neuronal cytoplasm and nerve terminals. α-Syn and GBA were expressed in both the striatum and cerebellum. However, the level of α-Syn in striatum was higher than that in cerebellum while the level of GBA in striatum was less than that in cerebellum. The activity of GBA was also lower in striatum than in cerebellum. Conclusion The expression levels of α-Syn and GBA are different in different brain regions. The level of α-Syn is negatively correlated with that of GBA.

Key words: α-synuclein, glucocerebrosidase, cynomolgus monkeys

CLC Number:

R338

MI Na, LIU Guangwei, LI Xin, LI Yaohua, YU Shun . Differential expressions of α-synuclein and glucocerebrosidase in different brain regions of cynomolgus monkeys[J]. Journal of Capital Medical University, 2013, 34(1): 110-114.

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URL: https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/10.3969/j.issn.1006-7795.2013.01.021

https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/Y2013/V34/I1/110

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