Effect of α-synuclein oligomer inhibit neurite outgrowth of rat primary cultured neuron

doi:10.3969/j.issn.1006-7795.2014.05.013

Abstract

Abstract:

Objective To investigate the effect of oligomerization of α-synuclein on the neurite outgrowth of brain neurons and indicate its function, explore the mechanism of Parkinson's disease. Methods Neurons isolated from the neocortex of newborn SD rats were cultured and corresponding proteins were added into the culture medium to observe the effect of the protein on the neurite outgrowth of the neurons. Added α-Syn oligomer, α-synuclein monomer into the culture system of neural cells, and compared the different promoting effect of neurite outgrowth with that of control groups. Western blotting assays and immunofluorescence staining assay were used to confirm whether the result is special. Observed with inverted phase contrast microscope,and photographs were applied to analyze with software.Results The mean neurite length of the neurons treated by α-Syn oligomer was significantly shorter than that of the control group neurons (P<0.05). But the length of neurons treated by α-Syn monomer is longer than that of the control group (P<0.05). Increased concentration of α-synuclein oligomer can enhance this effect. The result of Western blotting and Immunofluorescence staining assay showed that α-synuclein oligomer can enter the neural cell and inhibit the neurite outgrowth. Conclusion α-Synuclein can inhibit neurite outgrowth of primary cultured neuron and this is a dose-dependent effect.

Key words: α-synuclein, oligomer, primary neuronal culture, neurite outgrowth, Parkinson’s disease

CLC Number:

Wang Peng, Li Xin, Chen Yudong, Yang Weiwei, Li Xuran, Yu Shun. Effect of α-synuclein oligomer inhibit neurite outgrowth of rat primary cultured neuron[J]. Journal of Capital Medical University, 2014, 35(5): 587-591.

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URL: https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/10.3969/j.issn.1006-7795.2014.05.013

https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/Y2014/V35/I5/587

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