Alpha-synuclein promotes internalization of surface N-methyl-D-aspartate receptors in primary neurons and transgenic mice

doi:10.3969/j.issn.1006-7795.2014.05.015

Journal of Capital Medical University ›› 2014, Vol. 35 ›› Issue (5): 596-600.doi: 10.3969/j.issn.1006-7795.2014.05.015

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Alpha-synuclein promotes internalization of surface N-methyl-D-aspartate receptors in primary neurons and transgenic mice

Chen Yudong, Yang Weiwei, Li Xin, Wang Peng, Li Xuran, Yu Shun

Department of Neurobiology, Xuanwu Hospital, Capital Medical University; Laboratory of Molecular Diagnosis, Beijing Geriatric Medical Research Center; Key Laboratory of Neurodegenerative Disease, Ministry of Education, Beijing 100053, China

Received:2014-07-21 Online:2014-10-21 Published:2014-10-20
Supported by:
This study was supported by National Natural Science Foundation of China (81071014), National Basic Research and Development Program (973 Program) of China (2011CB504101), National Science and Technology Support Program (2012BAI10B03), Capital Health Research and Development Project (SHOUFA 2011-1001-01), Natural Science Foundation of Beijing (7102076).

Abstract

Abstract:

Objective To investigate the effect of α-synuclein (α-Syn) on surface N-methyl-D-aspartate (NMDA) receptors on the primary neurons and transgenic mice.Methods Immunofluorescent labeling and Western blotting analysis were used to measure the alterations of NMDA receptors and Rab5B proteins. Rab5B antisense oligonucleotides were applied to suppress Rab5B expression. The primary neurons and transgenic mice were used as models to observe the effect of α-Syn on the surface NMDAR expression.Results α-Syn triggered a significant decrease of the surface NMDAR expression. This effect of α-Syn was blocked by suppression of Rab5B expression. Conclusion α-Syn promotes internalization of surface NMDA receptors by increasing Rab5B expression.

Key words: α-synuclein, transgenic mice, primary neurons, N-methyl-D-aspartate, internalization

CLC Number:

Q189

Chen Yudong, Yang Weiwei, Li Xin, Wang Peng, Li Xuran, Yu Shun. Alpha-synuclein promotes internalization of surface N-methyl-D-aspartate receptors in primary neurons and transgenic mice[J]. Journal of Capital Medical University, 2014, 35(5): 596-600.

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Alpha-synuclein promotes internalization of surface N-methyl-D-aspartate receptors in primary neurons and transgenic mice

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