Establishment of transgenic mice over-expressing insulin-like growth factor binding protein-4 in the brain

doi:10.3969/j.issn.1006-7795.2015.04.018

Abstract

Abstract: Objective To establish a transgenic mouse which may over express insulin-like growth factor binding protein-4(IGFBP-4) in the brain, in order to explore the effect of IGFBP-4 on brain development. Methods The neuron-specific transgene expression cassette of IGFBP-4 was constructed and then microinjected into the fertilized mouse egg. The genotype of the transgenic mice was identified using PCR. The expression of IGFBP-4 protein in the brain was detected by Western blotting, and the spatial expression of IGFBP-4 was detected by immunohistochemistry. Both weights of body and brain of mice were measured. Results Exogenous IGFBP-4 cDNA was successfully integrated into the mouse genome and stably passaged. The expression of IGFBP-4 protein in the adult brain was increased by 267% in the transgenic mouse, while its spatial expression pattern was similar to that in the wild type mouse. The body weight was insignificantly changeable, but the whole brain weight was found an 8.4% increase in transgenic mice(P<0.05). Especially, the weight of brain stem increased by 16.5%(P<0.05), with no significant changes in other areas of the brain. Conclusion The exogenous IGFBP-4 cDNA was stably integrated into the transgenic mouse genome, and the over-expression of IGFBP-4 could affect development of certain part of the brain. The newly established IGFBP-4 transgenic mouse may therefore provide a useful animal model for investigating brain development.

Key words: insulin-like growth factor binding protein-4(IGFBP-4), transgenic mice, neurodevelopment

CLC Number:

Q786

Gou Rongbin, Jiang Xiaohua, Ju Rongkai, Niu Hanxu, Duan Deyi, Xu Qunyuan. Establishment of transgenic mice over-expressing insulin-like growth factor binding protein-4 in the brain[J]. Journal of Capital Medical University, 2015, 36(2): 262-269.

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URL: https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/10.3969/j.issn.1006-7795.2015.04.018

https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/Y2015/V36/I2/262

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