Impact of severe hypertriglyceridemia in mice on female reproduction and the lipid and glucose metabolism of their offsprings

doi:10.3969/j.issn.1006-7795.2014.01.012

Abstract

Abstract:

Objective Severe hypertriglyceridemia (HTG) accompanied with pregnancy may induce acute pancreatitis, which could result in adverse outcomes of pregnancy. However, the effect of maternal HTG on the metabolism of lipids and glucose in offsprings remains unclear. Using apolipoprotein CⅢ (Apo CⅢ) transgenic mice with severe HTG as model system, this study sought to investigate the effects of severe HTG on the reproduction of the female mice as well as on the metabolism of lipids and glucose of the offsprings.Methods Female HTG Apo CⅢ transgenic and normal mice were mated with normal males to observe the effect of severe HTG on fertility and obtained three groups offsprings: maternal transgenic but normal offspring (maternal control, MC), maternal transgenic and transgenic offspring (maternal transgenic, MT) and maternal normal and normal offspring (normal control, NC). The three groups of mice were compared for litter size, birth weight and growth rate, plasma triglycerides (TG), total cholesterol (TC) and differences in glucose metabolism.Results Severe HTG from Apo CⅢ transgene in female mice did not affect the reproduction with unchanged pregnancy and litter size. The pups from the 3 groups had similar body weight and growth rate. Influenced by Apo CⅢ transgene expression, the plasma TG and TC levels at 14 and 28 days were much higher in MT mice than those of MC and NC groups (P<0.01). MT mice also had mildly impaired glucose tolerance, higher fasting insulin levels and hence higher homeostasis model assessment insulin resistance index (HOMA-IR), compared with the other two groups (P<0.05).Conclusion Severe HTG Apo CⅢ transgenic female mice had normal pregnancy, parturition and lactation. The maternally inherited Apo CⅢ severe HTG transgenic mice after weaning exhibited abnormal glucose tolerance and insulin resistance.

Key words: apolipoprotein CⅢ(Apo CⅢ), transgenic mice, hypertriglyceridemia, insulin resistant, female reproduction

CLC Number:

R587.1

Ma Yahong, Abudula·Rexiti, Xie Beichen, Guo Xin, Yang Jinkui, Liu Guoqing. Impact of severe hypertriglyceridemia in mice on female reproduction and the lipid and glucose metabolism of their offsprings[J]. Journal of Capital Medical University, 2014, 35(1): 51-55.

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URL: https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/10.3969/j.issn.1006-7795.2014.01.012

https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/Y2014/V35/I1/51

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