Application of GPIHBP1 gene knockout mice in the study of lung injury induced by acute pancreatitis with severe hypertriglyceridemia

doi:10.3969/j.issn.1006-7795.2020.04.012

Abstract

Abstract: Objective To establish a model of acute pancreatitis in genetically modified mice with hypertriglyceridemia and to observe pathological changes of pancreas and lung tissues. Methods Wild type and glycosylphosphatidylinositol-anchored high-density lipoprotein binding protein 1(GPIHBP1) gene knockout mice with hypertriglyceridemia were used to induce acute pancreatitis via intraperitoneal injection of caerulein. Amylase activity and plasma levels of lipids were detected. Pathological changes of pancreas and lung tissues were observed. Results GPIHBP1 knockout mice showed extremely high plasma levels of triglycerides. Amylase activity was significantly altered after induction of acute pancreatitis. The pathological injury of pancreas was aggravated, accompanied by lung injury, and the cell number of alveolar lavage fluid significantly increased. Conclusion After induction of acute pancreatitis, the pathological damage of pancreas and lung tissues in GPIHBP1 knockout mice was significantly aggravated, indicating that it has certain significance for further exploring the pathogenesis of hyperlipidemic acute pancreatitis leading to lung and remote organ injury.

Key words: severe hypertriglyceridemia, acute pancreatitis, lung injury, GPIHBP1 gene knockout

CLC Number:

R657.5

Liu Xin, Xu Youqing, Cui Chunying, Zhao Zhigang. Application of GPIHBP1 gene knockout mice in the study of lung injury induced by acute pancreatitis with severe hypertriglyceridemia[J]. Journal of Capital Medical University, 2020, 41(4): 564-569.

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URL: https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/10.3969/j.issn.1006-7795.2020.04.012

https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/Y2020/V41/I4/564

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