Clinical importance of TRIM28 expression in gastric cancer and its effect on invasion and migration of gastric cancer cells

doi:10.3969/j.issn.1006-7795.2020.04.013

Abstract

Abstract: Objective To investigate the expression of tripartite motif-containing protein 28 (TRIM28) in gastric cancer (GC) tissues and cells and the relationship with clinical prognosis as well as its effect on the invasion and migration properties of GC cells and the underlying mechanisms. Methods The TCGA database and the quantitative real-time polymerase chain reaction (qRT-PCR) were used to analyze the expression level of TRIM28 in GC tissues and cells. The Kaplan-Meier Plotter database was applied to explore the relationship between TRIM28 expression and the clinicopathological features of GC patients. AGS GC cell line was used in this study, and the cell function was explored after TRIM28 knockdown with siRNA. The effects of TRIM28 on the invasion and migration of AGS cells were determined with Transwell assay and wound healing assay, respectively. The expressions of key proteins in Wnt/β-catenin signaling pathway were detected by Western blotting. Results The expression level of TRIM28 in GC tissues and cells was significantly higher. The overall survival of patients with high expression of TRIM28 were significantly lower than those with low expression of TRIM28. The invasion property of AGS cells was impaired after TRIM28 knockdown compared to the control siRNA-transfected cells (P<0.01). The results of wound healing assay showed that the migration ability of AGS cells was impaired after TRIM28 knockdown compared to the control siRNA-transfected cells (P<0.05). The expressions of β-catenin and c-Myc proteins were down-regulated with TRIM28 knockdown. Conclusion The expression of TRIM28 was increased in GC specimens. It promoted the invasion and migration of GC cells, associated with poor prognosis of the cancer.

Key words: gastric cancer, invasion, migration, tripartite motif-containing protein 28, Wnt/β-catenin signaling pathway

CLC Number:

R735.2

Ning Tingting, Xu Junxuan, Liu Si, Min Li, Zhu Shengtao. Clinical importance of TRIM28 expression in gastric cancer and its effect on invasion and migration of gastric cancer cells[J]. Journal of Capital Medical University, 2020, 41(4): 570-575.

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URL: https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/10.3969/j.issn.1006-7795.2020.04.013

https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/Y2020/V41/I4/570

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