Exploration of the action network and potential mechanism of Xiaochuanning Granule in prevention and treatment of psychological stress asthma based on network pharmacology

doi:10.3969/j.issn.1006-7795.2020.04.014

Abstract

Abstract: Objective To explore the action network and potential mechanism of Xiaochuanning Granule in the treatment of psychological stress asthma by the means of network pharmacology. Methods We screened the main effective components and targets of Xiaochuanning Granule on the data platforms such as Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform (TCMSP) and Bioinformatics Analysis Tool for Molecular Mechanism of Traditional Chinese Medicine (BATMAN-TCM) and studied the related targets of psychological stress asthma through the relevant disease target database. The common target of the drug and the disease was produced by Wayne diagram, and the drug-component-target interaction network was established by Cytoscape 3.7.2. Then the protein-protein interaction (PPI) network of common targets between compound and disease was analyzed with the STRING database. The key targets are screened by using plug-ins of Cytoscape 3.7.2 including MCODE, cytohubba and topology to screen. David database was used for Gene Ontology(GO) analysis and Kyoto Encyclopedia of Genes and Genomes(KEGG) pathway enrichment analysis. Results Totally 39 main active components, 121 predicted intersection targets of Xiaochuanning Granule were screened out. Totally 1063 psychological stress asthma targets were screened out, 72 common targets were achieved by Wayne diagram, crucial genes were calculated by cytohubba plug-in and topological operation, and gene interaction modules were screened out by MCODE. Therefore, the intersection Targets were obtained such as MAPK1, MAPK8, TNF, VEGFA, EGF, PTGS2, JUN, IL-6 and so on. GO and KEGG enrichment analysis suggested these targets were involved in biological processes such as signal transduction, positive regulation of cell proliferation, and activation of MAPK activity, etc. These common targets involve signaling pathways such as neuroactive ligand-receptor interaction, TNF, NOD-like receptor, Toll-like receptor, calcium signaling pathway, T cell receptor, PI3K-Akt and so on. Conclusion Xiaochuanning Granule may prevent and treat psychological stress asthma via the regulation of the "neuro-endocrine-immune network" through the nervous mechanism, immune inflammation, cell proliferation and other aspects.

Key words: network pharmacology, Xiaochuanning Granule, psychological stress asthma, potential mechanism, neuro-endocrine-immune network

CLC Number:

R256.1

Gong Xuefeng, Ren Peizhong, Cui Hongsheng, Jiang Zhenyuan, Zhang Shiyu, Wang Jiamei, Zhang Xinyu, Chen Qiuyi, Lyu Mingsheng, Hou Dan. Exploration of the action network and potential mechanism of Xiaochuanning Granule in prevention and treatment of psychological stress asthma based on network pharmacology[J]. Journal of Capital Medical University, 2020, 41(4): 576-589.

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URL: https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/10.3969/j.issn.1006-7795.2020.04.014

https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/Y2020/V41/I4/576

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