A network pharmacology approach to uncover the molecular mechanisms of Houshiheisan for treatment of ischemic stroke

doi:10.3969/j.issn.1006-7795.2021.01.008

Abstract

Abstract: Objective To analyze the core components and possible molecular mechanisms of Houshiheisan in the treatment of cerebral ischemia by network pharmacological method. Methods Through active components screening, components targets and disease targets prediction of Houshiheisan, the “component-target” network was established and analyzed to obtain the effective components and targets. Protein-protein interaction(PPI) network was constructed for Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis to identify potential signal pathways. Results Five effective components of Houshiheisan against cerebral ischemia, including β-Sitosterol, cryptopine, fumarine, stigmasterol, and (2R)-7-hydroxy-5-methoxy-2-phenylchroman-4-one, and 22 important anti-cerebral ischemia targets, including estrogen receptor(ESR1), androgen receptor(AR), prostaglandin G/H synthase 2(PTGS2), prothrombin(F2), nitric oxide synthase, endothelial(NOS3), etc. were found. Based on the PPI results, pharmacological targets of Houshiheisan treating cerebral ischemia might have a close relationship with the fluid shear stress pathway, advanced glycation end products-receptor for advanced glycation end products (AGE-RAGE) pathway, and hypoxia inducible factor1-α/vascular endothelial growth factor(HIF1-α/VEGF) pathway, etc. Conclusion Houshiheisan for treatment of ischemic stroke mainly plays a role in regulating inflammation, angiogenesis, and oxidative stress. It holds direct or indirect relationships with multiple signal pathways and has the characteristics of multi-components, multi-targets, and multi-pathways.

Key words: network pharmacology, Houshiheisan, ischemic stroke, molecular mechanism

CLC Number:

R229

Wang Xuan, Ma Chongyang, Zhang Yawen, Cheng Hongfa, Zhang Qiuxia. A network pharmacology approach to uncover the molecular mechanisms of Houshiheisan for treatment of ischemic stroke[J]. Journal of Capital Medical University, 2021, 42(1): 43-52.

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URL: https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/10.3969/j.issn.1006-7795.2021.01.008

https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/Y2021/V42/I1/43

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