Effects of chemical components of HSHS on the balance of inflammatory cytokines in LPS-stimulated BV2 cells

doi:10.3969/j.issn.1006-7795.2017.02.013

Abstract

Abstract: Objective To investigate the influence of chlorogenic acid, cynaroside, luteolin, ginsenoside Rg1 on expression of inflammatory cytokines. Methods The experiment was divided into six groups:control, model, chlorogenic acid, cynaroside, luteolin and ginsenoside Rg1 groups. The mimic ischemia injured microglia model was induced by LPS. The cyto-activity was detected via cell count kit. The NO content was determined by Griess Reagent. Contents of TNF-α、IL-6、IL-10 and TGF-β₁ were detected by ELISA.The expression levels of p-p65 and p-IκBα were detected by Western blotting. Results Compared with those results of model group, chlorogenic acid, cynaroside, luteolin and ginsenoside Rg1 groups could significantly inhibit the release of NO, and decrease the content of TNF-α and IL-6, ginsenoside Rg1 markedly elevated the TGF-β₁ level without influencing the cell survival. Chlorogenic acid, cynaroside, luteolin and ginsenoside Rg1 groups could decrease the expression of p-p65, p-IκBα. Conclusion The findings demonstrated that chlorogenic acid, cynaroside, luteolin and ginsenoside Rg1 played regulating roles in balancing ischemia injured microglia homeostasis via promoting anti-inflammatory cytokines as well as inhibiting the inflammatory cytokines. The therapeutic roles in the inflammatory reaction of cerebral ischemia which perhaps worked through the activation of NF-κB signaling pathway.

Key words: Houshiheisan, chlorogenic acid, cynaroside, luteolin, ginsenoside Rg1, microglia, inflammatory cytokines, NF-κB signaling pathway

CLC Number:

R289.5

Chang Jiahui, Zhao Hui, Wang Lei, Zhang Chi, Lu Yue, Zhang Qiuxia. Effects of chemical components of HSHS on the balance of inflammatory cytokines in LPS-stimulated BV2 cells[J]. Journal of Capital Medical University, 2017, 38(2): 213-219.

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URL: https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/10.3969/j.issn.1006-7795.2017.02.013

https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/Y2017/V38/I2/213

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