CD200R1 deletion promotes LPS-induced endocytosis of microglia

doi:10.3969/j.issn.1006-7795.2022.01.015

Abstract

Abstract: Objective To study the effect of CD200R1 on the endocytosis of microglia induced by bacterial lipopolysaccharide (LPS). Methods The changes of microglia endocytosis level was observed with fluorescentmicrobeads endocytosis experiment, and the expression of CD200R1mRNA was detected with RT-qPCR, and also it was observed in the BV2-SH-SY5Y co-culture model. Moreover, the primary microglia was extracted from CD200R1 knockout mice to observe the changes of the endocytic function induced by LPS. Results LPS activated microglia and promoted the increase of endocytosis and the decrease of CD200R1 expression in microglia. In the BV2-SH-SY5Y co-culture model, the neuronal cells contacted microglia to repress the increase of LPS-induced microglia endocytosis. The level of endocytosis increased more significantly in the CD200R1 knockout microglia treated with LPS. Conclusion CD200R1 knockout microglia showed more significant increase in endocytosis after LPS stimulation.

Key words: CD200R1, microglia, BV2 cell line, SH-SY5Y cell line, lipopolysaccharide, endocytosis

CLC Number:

R329.2
R741

Du Tianshu, Gong Xiaoli, Aizier Ainiwaer, Zhang Zhen, Liu Yang, Wang Xiaomin, Zhang Ting. CD200R1 deletion promotes LPS-induced endocytosis of microglia[J]. Journal of Capital Medical University, 2022, 43(1): 82-90.

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URL: https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/10.3969/j.issn.1006-7795.2022.01.015

https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/Y2022/V43/I1/82

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