Changes in cognition, emotion and microglia condition caused by chronic cerebral ischemia in rats

doi:10.3969/j.issn.1006-7795.2014.06.013

Abstract

Abstract:

Objective To observe the cognition, emotion, tissue condition and microglia condition after chronic cerebral ischemia caused by bilateral common carotid artery(BCCA) occlusion on 42nd and 56th day, and provide the basis to understand forming time of chronic cerebral ischemic model. Methods Sprague-Dawley(SD) rats were divided into 4 groups by sortition randomization method: 42 d-Sham, 42 d-Model, 56 d-Sham and 56 d-Model. Morris water maze, object location test and open field test were measured to evaluate cognition and emotion. To observe tissue condition of hippocampus, we chose H&E staining. The microglia activity was measured by Iba1 immunofluorescent staining(IF). Results The result of morris water maze showed that compared with the Sham group, the cognition of Model group was decreased, the decrease was more significant in 56 d-Model. Model group showed that the escape latency was longer, and the frequency of platform crossing was reduced. The result of object location test also showed the cognition of Model group was decreased. The discrimination index and explore time of new object was reduced. But there were no significant difference between them. The open field test showed vertical number and crossing number of Model group increased clearly, indicating that Model group had anxiety behavior. The H&E staining result showed that there were no changes in hippocampus tissue condition in the four groups. The IF staining showed that there were significant differences between the Model group and Sham group in Iba1 protein expression. Number of microglia in Model group was higher, colour was deepening, the cell was bigger, and the protuberances were increased and shortened, the protuberances like amoebocyte. Conclusion The cognition of rats was reduced at 42 days after BCCA operation, and the microglia were activated. It indicated that we can choose 42 day as forming time of chronic cerebral ischemia model. According to the cognition of 56 d-Model was worse than 42 d-Model, we suspect that the change is more significant with time. But we should notice that there were no changes in tissue condition at 42 days and 56 days described as HE staining.

Key words: chronic cerebral ischemia, cognition, emotion, microglia

CLC Number:

R734.2

Wang Liye, Lin Can, Zhao Haiping, Zhang Chencheng, Yan Feng, Chen Zhigang, Wang Rongliang, Wang Ningqun, Luo Yumin. Changes in cognition, emotion and microglia condition caused by chronic cerebral ischemia in rats[J]. Journal of Capital Medical University, 2014, 35(6): 741-747.

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URL: https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/10.3969/j.issn.1006-7795.2014.06.013

https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/Y2014/V35/I6/741

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