Effect of local intraarterial infusion of low-dose erythropoietin in combination with tissue plasminogen activator on focal cerebral ischemia in rats

doi:10.3969/j.issn.1006-7795.2014.06.014

Abstract

Abstract:

Objective To investigate the effect of local intraarterial infusion of low-dose erythropoietin(EPO) in combination with tissue plasminogen activator(tPA) on focal cerebral ischemic stroke. Methods Adult male Sprague-Dawley rats were subjected to middle cerebral artery occlusion for 2 or 4 h, followed by a tPA infusion over 30 min at the onset of reperfusion, with or without EPO(800 IU/kg) infusion via middle cerebral artery. A total of 50 male rats were randomly divided into 5 groups. Results Local intraarterial infusion of low-dose EPO in combination with tPA protected against transient focal cerebral ischemia in rats, manifested by a decrease in mortality, neurological deficit scores, brain edema and hemorrhage with no hematological side effects after 24 h reperfusion. The levels of p-AKT and p-ERK were both increased in response to administration of EPO, suggesting a concurrent activation of AKT and ERK pathways. Conclusion Local intraarterial infusion of low-dose EPO in combination with tPA protected against focal cerebral ischemia in rats with no significant side effects, involving activation of AKT and ERK pathways.

Key words: erythropoietin, tissue plasminogen activator, neuroprotection, cerebral ischemia, reperfusion

CLC Number:

R743.31

Wang Rongliang, Zhao Haiping, Wu Xiaoning, Gao Jinhuan, Ji Xunming, Luo Yumin. Effect of local intraarterial infusion of low-dose erythropoietin in combination with tissue plasminogen activator on focal cerebral ischemia in rats[J]. Journal of Capital Medical University, 2014, 35(6): 748-754.

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URL: https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/10.3969/j.issn.1006-7795.2014.06.014

https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/Y2014/V35/I6/748

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