Changes of Pbk and its correlation with cytokine IGF-1 in glial cells after oxygen and glucose deprivation

doi:10.3969/j.issn.1006-7795.2021.02.015

Abstract

Abstract: Objective To investigate the changes of PDZ-binding-kinase(Pbk) and its correlation with cytokine insulin-like growth factors-1(IGF-1) in glial cells at different time points after oxygen and glucose deprivation.Methods BV2 microglia cells were cultured, primary astrocytes were isolated, and the oxygen and glucose deprivation(OGD) model was prepared. The expressions of cytokines IGF-1 and signal molecules Pbk and HDAC2 in two kinds of glia cells after 1, 3 and 6 h of oxygen and glucose deprivation were observed by reverse transcription-polymerase chain reaction(RT-PCR). Pearson correlation analysis of cytokines and signal molecules was carried out. Results With the time extension of OGD, the transcription level of Pbk was increased firstly and then decreased in microglia cells, and gradually increased in astrocytes, with statistically significant differences between groups (P<0.05). The transcription level of HDAC2 was decreased gradually in microglia cells and increased gradually in astrocytes, with statistically significant differences between groups (P<0.05). The transcription level of IGF-1 was decreased gradually in microglia cells and increased gradually in astrocytes, with statistically significant differences between groups (P<0.05).The transcription levels of Pbk and HDAC2 in microglia cells and astrocytes were positively correlated with the transcription level of IGF-1 after oxygen and glucose deprivation (P<0.05).Conclusion During the early stage of hypoxic-ischemic injury, the secretion of IGF-1 decreased in microglia and increased in astrocyte, which may be related to the activation of the Pbk/HDAC2/IGF-1 pathway. It provides a basis for further research on the neuroprotective mechanism after ischemia.

Key words: ischemia and hypoxia, microglia, astrocyte, cytokines, PDZ binding kinase

CLC Number:

Q421

Wang Yuqing, Chen Zhigang, Li Fangfang, Zhang Sijia, Luo Yumin, Zhao Haiping. Changes of Pbk and its correlation with cytokine IGF-1 in glial cells after oxygen and glucose deprivation[J]. Journal of Capital Medical University, 2021, 42(2): 251-256.

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URL: https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/10.3969/j.issn.1006-7795.2021.02.015

https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/Y2021/V42/I2/251

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