Reduced expression of cholinergic neuronal marker in the dorsal motor nucleus of the vagus and constipation are observed in the rats with lipopolysaccharide microinjection in bilateral substantia nigra

doi:10.3969/j.issn.1006-7795.2013.04.017

Abstract

Abstract:

Objective To study the alteration of the distribution of tyrosine hydroxylase(TH) and choline acetyltransferase(ChAT) immunoreactivity-positive neurons and nerve fibers in the dorsal motor nucleus of the vagus(DMV) in rats with lipopolysaccharide(LPS) microinjection of substantia nigra(SN). Methods Rats were subjected to LPS in bilateral SN to make the LPS Parkinson's disease rat model(LPS rats). Immunofluorescence were used in this study. Results In the rats treated with LPS, the neurons' number in SN was markedly reduced accompanied by abundant proliferation of glial cells, and the TH immunoreactivity(IR) in SN was obviously reduced. However, the TH-IR neurons were significantly increased, and the ChAT-IR significantly reduced in DMV. Furthermore, the rats treated with LPS showed obvious constipation with reduced weight and water content in the stool. Conclusion Injection of LPS into substantia nigra can cause a constipation in rats, which may be related to the enhanced TH-IR and reduced ChAT-IR in DMV leading to gastrointestinal dysmotility in LPS treated rats.

Key words: lipopolysaccharide, Parkinson’s disease model, constipation, dorsal motor nucleus of the vagus, choline acetyltransferase

CLC Number:

R333.3

ZHANG Yue, ZHENG Lifei, SONG Jin, FAN Ruifang, CHEN Changliang, ZHU Jinxia. Reduced expression of cholinergic neuronal marker in the dorsal motor nucleus of the vagus and constipation are observed in the rats with lipopolysaccharide microinjection in bilateral substantia nigra[J]. Journal of Capital Medical University, 2013, 34(4): 566-571.

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URL: https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/10.3969/j.issn.1006-7795.2013.04.017

https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/Y2013/V34/I4/566

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