Journal of Capital Medical University ›› 2013, Vol. 34 ›› Issue (4): 572-575.doi: 10.3969/j.issn.1006-7795.2013.04.018

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Effects of Zhuhonggao on the expression of cytokines and adhesion molecule in THP-1 cells induced by lipopolysaccharide

HE Xiujuan1,2, LIN Yan2, LV Zhe1, AN Yunqing1, DING Yuezhong1, LI Ping2   

  1. 1. Department of Immunology, Capital Medical University, Beijing 100069, China;
    2. Department of Pathophysiology, Beijing Institute of Traditional Chinese Medicine, Beijing Hospital of Traditional Chinese Medicine, Capital Medical University, Beijing 100010, China
  • Received:2013-02-21 Online:2013-08-21 Published:2013-07-20
  • Supported by:

    This study was supported by Natural Science Foundation of Capital Medical University(2011ZR03).

Abstract:

Objective To observe the effects of Zhuhonggao(ZHG) on the expression of interleukin 1 beta(IL-1β), soluble intercellular adhesion molecule-1(sICAM-1), interleukin-8(IL-8) and monocyte chemoattractant protein-1(MCP-1) induced by lipopolysaccharide(LPS) in THP-1 cells, and study the mechanisms of ZHG on promoting skin wound healing. Methods THP-1 cell activity was observed by Cell Counting Kit-8(CCK-8) after treating with ZHG. The expression of IL-1β, sICAM-1, IL-8 and MCP-1 were measured by enzyme linked immunosorbent assay(Elisa). Results After treating with ZHG and LPS for 24 h, ZHG decreased the expression of MCP-1(content of mercury, Hg 4.2~8.4 mg/kg)(P<0.01, P<0.05), decreased the expression of IL-1β(Hg 2.1 mg/kg)(P<0.05) and IL-8(Hg 2.1~8.4 mg/kg)(P<0.01, P<0.05). ZHG had no significant effect on sICAM-1. Conclusion It is possible that ZHG inhibit the expression of IL-1β, IL-8 and MCP-1 on THP-1 cells, therefore may inhibit excessive inflammatory response.

Key words: wound healing, Zhuhonggao, adhesion molecule, chemokine, inflammatory factor

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