Effects of Zhuhonggao on the expression of cytokines and adhesion molecule in THP-1 cells induced by lipopolysaccharide

doi:10.3969/j.issn.1006-7795.2013.04.018

Abstract

Abstract:

Objective To observe the effects of Zhuhonggao(ZHG) on the expression of interleukin 1 beta(IL-1β), soluble intercellular adhesion molecule-1(sICAM-1), interleukin-8(IL-8) and monocyte chemoattractant protein-1(MCP-1) induced by lipopolysaccharide(LPS) in THP-1 cells, and study the mechanisms of ZHG on promoting skin wound healing. Methods THP-1 cell activity was observed by Cell Counting Kit-8(CCK-8) after treating with ZHG. The expression of IL-1β, sICAM-1, IL-8 and MCP-1 were measured by enzyme linked immunosorbent assay(Elisa). Results After treating with ZHG and LPS for 24 h, ZHG decreased the expression of MCP-1(content of mercury, Hg 4.2~8.4 mg/kg)(P<0.01, P<0.05), decreased the expression of IL-1β(Hg 2.1 mg/kg)(P<0.05) and IL-8(Hg 2.1~8.4 mg/kg)(P<0.01, P<0.05). ZHG had no significant effect on sICAM-1. Conclusion It is possible that ZHG inhibit the expression of IL-1β, IL-8 and MCP-1 on THP-1 cells, therefore may inhibit excessive inflammatory response.

Key words: wound healing, Zhuhonggao, adhesion molecule, chemokine, inflammatory factor

CLC Number:

HE Xiujuan, LIN Yan, LV Zhe, AN Yunqing, DING Yuezhong, LI Ping. Effects of Zhuhonggao on the expression of cytokines and adhesion molecule in THP-1 cells induced by lipopolysaccharide[J]. Journal of Capital Medical University, 2013, 34(4): 572-575.

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URL: https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/10.3969/j.issn.1006-7795.2013.04.018

https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/Y2013/V34/I4/572

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