Effect of dexamethasone on the chemokine production in nasal polyps in vitro

doi:10.3969/j.issn.1006-7795.2024.02.010

Journal of Capital Medical University

Previous Articles Next Articles

Effect of dexamethasone on the chemokine production in nasal polyps in vitro

Meng Haiyang^1,2,3, Wang Min^1,2, Li Ying^1,2, Wang Xiangdong^1,2*#, Zhang Luo^1,2*#

1.Department of Otolaryngology Head and Neck Surgery and Department of Allergy, Beijing Tongren Hospital, Capital Medical University,Beijing 100730, China； 2.Beijing Institute of Otolaryngology, Beijing Institute of Integrated Traditional Chinese and Western Medicine in Otolaryngology, Beijing Laboratory of Allergic Diseases (Beijing Municipal Education Commission), Beijing Key Laboratory of Nasal Diseases, Key Laboratory of Otolaryngology Head and Neck Surgery (Capital Medical University)，Ministry of Education, Beijing 100005, China ； 3.Department of Otolaryngology Head and Neck Surgery, Beijing Renhe Hospital, Beijing 102600, China

Received:2023-01-18 Online:2024-04-25 Published:2024-04-25
Supported by:
This study was supported by National Key Research and Development Program (2022YFC2504100), National Natural Science Foundation of China(81570893,81970852,82171110). Changjiang Scholars Innovation Team of the Ministry of Education (IRT13082), Medical and Health Science and Technology Innovation Project of the Chinese Academy of Medical Sciences (2019-I2M-5-022), Natural Science Foundation of Beijing(7162042, 7222024), Capital Clinical Characteristic Diagnosis and Treatment Technology Research and Transformation Application Key Project (Z211100002921057), Capital Health Development Scientific Research Key Project (2022-1-1091), Reform and Development Project of Public Institutions (2019-10), New Star Cross Cooperation Project (20220484226), Leading Talents in Beijing Health System High-level Public Health Talent Construction Project (Lingjunrencai-01-08).

Abstract

Abstract: Objective To observe the effect of dexamethasone (DEX) on the production of chemokines in nasal polyp tissue of patients with chronic sinusitis with nasal polyps (CRSwNP). Methods Dispersed nasal polyp cells were prepared from nasal polyp tissues of CRSwNP patients and then the cells were treated with 10, 50 and 100 μmol/L DEX. The cell viability and levels of chemokines in cell culture supernatant were detected 24 h after DEX treatments. Results Compared to the untreated group, the percentage of viable cells in the 10, 50 and 100 μmol/L DEX-treated groups were unchanged. The levels of CCL2, CCL11, CXCL2 and CXCL9 were significantly lower, CCL20 significantly higher, CCL3,CCL4, CCL5, CCL17 and CXCL5 unchanged.Conclusion DEX reduced the production of multiple chemokines in dispersed nasal polyp cells of nasal polyp tissue, which may inhibit infiltration of inflammatory cells and occurrence of inflammatory response.

Key words: chronic sinusitis with nasal polyps, dexamethasone, chemokine

CLC Number:

R765

Meng Haiyang, Wang Min, Li Ying, Wang Xiangdong, Zhang Luo. Effect of dexamethasone on the chemokine production in nasal polyps in vitro[J]. Journal of Capital Medical University, doi: 10.3969/j.issn.1006-7795.2024.02.010.

References

［1］ Schleimer R P. Immunopathogenesis of chronic rhinosinusitis and nasal polyposis［J］. Annu Rev Pathol, 2017, 12: 331-357.
［2］ Wang X D, Sima Y T, Zhao Y, et al. Endotypes of chronic rhinosinusitis based on inflammatory and remodeling factors［J］. J Allergy Clin Immunol, 2023, 151(2): 458-468.
［3］ Hughes C E, Nibbs R J B. A guide to chemokines and their receptors［J］. FEBS J, 2018, 285(16): 2944-2971.
［4］李颖, 王向东, 王鸿, 等. CC类趋化因子在慢性鼻窦炎的表达［J］. 首都医科大学学报, 2013, 34(6): 795-798.
［5］ De Corso E, Baroni S, Battista M, et al. Nasal fluid release of eotaxin-3 and eotaxin-2 in persistent sinonasal eosinophilic inflammation［J］. Int Forum Allergy Rhinol, 2014, 4(8): 617-624.
［6］ Wang C, Lou H, Wang X, et al. Effect of budesonide transnasal nebulization in patients with eosinophilic chronic rhinosinusitis with nasal polyps［J］. J Allergy Clin Immunol, 2015, 135(4): 922-929.
［7］ Fokkens W J, Lund V J, Hopkins C, et al. European position paper on rhinosinusitis and nasal polyps 2020［J］. Rhinology, 2020, 58(Suppl 29): 1-464.
［8］ Wang M, Zhang N, Zheng M, et al. Cross-talk between TH2 and TH17 pathways in patients with chronic rhinosinusitis with nasal polyps［J］. J Allergy Clin Immunol, 2019, 144(5): 1254-1264.
［9］ Zeng M, Li Z Y, Ma J, et al. Clarithromycin and dexamethasone show similar anti-inflammatory effects on distinct phenotypic chronic rhinosinusitis: an explant model study［J］. BMC Immunol, 2015, 16: 37.
［10］ Locati M, Curtale G, Mantovani A. Diversity, mechanisms, and significance of macrophage plasticity［J］. Annu Rev Pathol, 2020, 15: 123-147.
［11］ Shapouri-Moghaddam A, Mohammadian S, Vazini H, et al. Macrophage plasticity, polarization, and function in health and disease［J］. J Cell Physiol, 2018, 233(9): 6425-6440.
［12］ Banks C A, Schlosser R J, Wang E W, et al. Macrophage infiltrate is elevated in CRSwNP sinonasal tissue regardless of atopic status［J］. Otolaryngol Head Neck Surg, 2014, 151(2): 215-220.
［13］ Krysko O, Holtappels G, Zhang N, et al. Alternatively activated macrophages and impaired phagocytosis of S. aureus in chronic rhinosinusitis［J］. Allergy, 2011, 66(3): 396-403.
［14］ Wang X, Zhang N, Bo M, et al. Diversity of TH cytokine profiles in patients with chronic rhinosinusitis: a multicenter study in Europe, Asia, and Oceania［J］. J Allergy Clin Immunol, 2016, 138(5): 1344-1353.
［15］ Gevaert P, Han J K, Smith S G, et al. The roles of eosinophils and interleukin-5 in the pathophysiology of chronic rhinosinusitis with nasal polyps［J］. Int Forum Allergy Rhinol, 2022, 12(11): 1413-1423.
［16］ Delemarre T, Holtappels G, De Ruyck N, et al. A substantial neutrophilic inflammation as regular part of severe type 2 chronic rhinosinusitis with nasal polyps［J］. J Allergy Clin Immunol, 2021, 147(1): 179-188.e2.
［17］ Wen W P, Liu W L, Zhang L, et al. Increased neutrophilia in nasal polyps reduces the response to oral corticosteroid therapy［J］. J Allergy Clin Immunol, 2012, 129(6): 1522-1528.e5.
［18］ Wang L, Yang M, Wang X, et al. Glucocorticoids promote CCL20 expression in keratinocytes［J］. Br J Dermatol, 2021, 185(6): 1200-1208.
［19］ Zijlstra G J, Fattahi F, Rozeveld D, et al. Glucocorticoids induce the production of the chemoattractant CCL20 in airway epithelium［J］. Eur Respir J, 2014, 44(2): 361-370.

[1]	Yang Jingxing, Zhao Yan, Chen Luquan, Cai Chao#, Wang Xiangdong, #. he mechanism of Scutellaria baicalensis in treatment of chronic rhinosinusitis based on network pharmacology [J]. Journal of Capital Medical University, 0, (): 252-261.
[2]	Zheng Ming , Wu Jianyou , Sima Yutong, Wang Xiangdong, Zhang Luo, . Comparison of clinical characteristics between odontogenic sinusitis patients with and without sinusitis symptoms [J]. Journal of Capital Medical University, 2024, 45(2): 239-245.
[3]	Wang Ming, Hong Yu, Bu Xiangting, Wang Chengshuo, Zhang Luo. Dysregulation of genes related to fibrin formation and degradation in nasal mucosa of patients with chronic rhinosinusitis with nasal polyps [J]. Journal of Capital Medical University, 2024, 45(2): 246-251.
[4]	Feng Ying, Shi Ke, Wang Xianbo. Establishment and validation of 2-year predictive scoring model for patients with liver cirrhosis complicated with superior mesenteric venous thrombosis [J]. Journal of Capital Medical University, 2023, 44(1): 107-114.
[5]	Li Yan, Wang Qiqi, Zhang Yuling, Duan Su, Zhang Haibo, Wang Xiangdong, Lan Feng. Clinical characteristics of chronic rhinosinusitis with nasal polyps with different body mass index [J]. Journal of Capital Medical University, 2022, 43(6): 899-904.
[6]	Yang Jingxing, Cai Chao, Zhao Yan, Wang Xiangdong, Zhang Luo. Research advances in the anti-inflammatory effects and mechanisms of baicalin in chronic inflammatory diseases of respiratory mucosa [J]. Journal of Capital Medical University, 2022, 43(6): 919-923.
[7]	Wang Qiqi, Lan Feng, Wang Jing, Hao Liusiqi, Li Yan, Zhang Luo. The effects of PCDH9 on function of nasal epithelial barrier in vitro in allergic rhinitis [J]. Journal of Capital Medical University, 0, (): 886-892.
[8]	Zhao Zhiyue, Zhang Luo. From China to the world, Professor Zhang Luo's inspiration journey in chronic nasal diseases [J]. Journal of Capital Medical University, 2020, 41(5): 742-747.
[9]	Wang Min, Li Ying, Wang Xiangdong, Zhang Luo. Expression and role of IL-23 in chronic rhinosinusitis [J]. Journal of Capital Medical University, 2020, 41(2): 178-182.
[10]	Wang Ming, Bu Xiangting, Luan Ge, Wang Yang, Wang Chengshuo, Zhang Luo. Expression and glucocorticoid regulation of MALAT1 in chronic rhinosinusitis with nasal polyps [J]. Journal of Capital Medical University, 2020, 41(2): 183-188.
[11]	Du Kun, Zheng Ming, Wang Xiangdong, Zhang Luo. Analysis for the relationship between interleukin-21 and tissue IgE in recurrent nasal polyps [J]. Journal of Capital Medical University, 2020, 41(2): 194-199.
[12]	Zhang Peng, Yang Wei, Wang Kuiji, Tan Yi, Shang Xiaojuan, Shu Ning, Wang Xiang, Xu Shiwen, Sun Sanfeng, Zhao Xinyu, Yu Kun, Chen Luquan. A multi-center prospective case series study on the treatment of allergic rhinitis by acupuncture of sphenopalatine ganglion [J]. Journal of Capital Medical University, 2019, 40(6): 916-920.
[13]	Meng Xianglong, Hai Yong, Yang Jincai, Su Qingjun. Retrospective study of cervical dynamic stabilization in the treatment of single level cervical spondylosis [J]. Journal of Capital Medical University, 2019, 40(1): 124-128.
[14]	Xu Qingqing, Duan Su, Wang Xiangdong, Zhang Luo. Meta-analysis of the effect of the correction of deviation of the nasal septum in patients with allergic rhinitis [J]. Journal of Capital Medical University, 2018, 39(6): 928-932.
[15]	Gao Zhanmei, Song Yang, Liu Hua, Wen Changlong, Sun Haiyang. Rapeutic effect of azelastine nasal spray combined with desloratadine in the treatment of allergic rhinitis: a system review and Meta-analysis [J]. Journal of Capital Medical University, 2018, 39(3): 458-464.

Effect of dexamethasone on the chemokine production in nasal polyps in vitro

PDF

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments