The effects of PCDH9 on function of nasal epithelial barrier in vitro in allergic rhinitis

doi:10.3969/j.issn.1006-7795.2022.06.011

Abstract

Abstract: Objective To investigate the effect of protocadherin 9 (PCDH9) on function of nasal epithelial barrier in allergic rhinitis (AR). Methods The microarray data was downloaded from GEO dataset to analysis the nasal mucosa different expression genes between AR patients and control, and functional enrichment analysis of differentially expressed genes was performed to screen the hub genes. Further, nasal mucosa epithelial cells from AR patients and controls were collected to verify the hub gene expression level. PCDH9 was overexpressed or knocked down in human nasal epithelial cells (HNEPC) and bronchial epithelial cells (BEAS-2B) to identify the effect of PCDH9 on tight junction and Wnt pathway activation. Results There were 4 406 differentially expressed genes between nasal mucosa of AR and control group in GSE118243 datasets, which were mainly involved in cell adhesion biological processes. PCDH9 was both involved in Wnt signaling pathway and cell adhesion. The expression of PCDH9 was significantly upregulated in AR nasal epithelial cells compared with control by quantitative polymerase chain reaction (Q-PCR). It was further demonstrated in cellular study that PCDH9 enhanced ZO-1 mRNA and protein expression and inhibited β-catenin protein expression in nasal epithelial cells. Conclusion PCDH9 was increased in nasal mucosa of AR patients, which can promote the expression of tight junction and inhibit the activation level of Wnt signaling pathway in vitro.

Key words: allergic rhinitis, epithelial barrier, protocadherin, tight junction

CLC Number:

R765

Wang Qiqi, Lan Feng, Wang Jing, Hao Liusiqi, Li Yan, Zhang Luo. The effects of PCDH9 on function of nasal epithelial barrier in vitro in allergic rhinitis[J]. Journal of Capital Medical University, 0, (): 886-892.

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