Effect of transforming growth factor-β1 on tight junction barrier function in nasal epithelial cells of chronic rhinosinusitis with nasal polyps

doi:10.3969/j.issn.1006-7795.2018.01.009

Journal of Capital Medical University ›› 2018, Vol. 39 ›› Issue (1): 45-50.doi: 10.3969/j.issn.1006-7795.2018.01.009

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Effect of transforming growth factor-β1 on tight junction barrier function in nasal epithelial cells of chronic rhinosinusitis with nasal polyps

Jiao Jian¹, Zhang Luo^1,2

1. Department of Otorhinolaryngology Head and Neck Surgery, Beijing Tongren Hospital, Capital Medical University, Beijing Institute of Otorhinolaryngology, Key Laboratory of Otorhinolaryngology Head and Neck Surgery, Ministry of Education, Beijing Key Laboratory of Nasal Diseases, Beijing 100730, China;
2. Department of Allergy, Beijing Tongren Hospital, Capital Medical University, Beijing 100730, China

Received:2017-12-11 Online:2018-01-21 Published:2018-01-27
Supported by:
This study was supported by National Natural Science Foundation of China (81420108009,81630023),Changjiang Scholars and Innovative Research Team (IRT13082), Beijing Talent Project (2016), Natural Science Foundation of Beijing (7172053), Beijing Health Bureau Program for High Level Talents (2009-2-007, 2011-3-043, and 2014-3-018), Beijing Municipal Administration of Hospitals' Mission Plan (SML20150203), Beijing Scientific and Technological Overall Plan (Z171100000117002).

Abstract

Abstract: Objective To investigate the epithelial tight junction (TJ) barrier integrity changes and its regulation by transforming growth factor(TGF)-β1 in Chinese patients with chronic rhinosinusitis with nasal polyps(CRSwNP).Methods Air-liquid interface(ALI) cultures were established for human nasal epithelial cells from CRSwNP patients and healthy control subjects. Differentiated cells were stimulated with different concentrations of TGF-β1, transepithelial resistance(TER) and permeability of FITC-Dextran was measured over time. Additionally, TJ protein expression was examined by using immunofluorescence and TJ mRNA expression was determined by real-time PCR.Results In comparison with control subjects, epithelial TER decreased and FITC-Dextran permeability increased in CRSwNP-derived epithelial cells along with an irregular, patchy, and decreased expression of the TJ proteins occludin and zonula occludens(ZO)-1. 10 ng/mL TGF-β1 stimulation decreased epithelial TER, increased paracellular permeability, as well as disrupted TJ protein occludin and ZO-1 expression pattern in both control and CRSwNP-derived epithelial cells.Conclusion Epithelial TJ barrier integrity was disrupted in Chinese CRSwNP patients. TGF-β1 played an important role in inducing TJ barrier defects in nasal epithelium, suggesting that it might serve as a potential therapeutic target for CRSwNP treatment.

Key words: nasal polyp, tight junction, transforming growth factor-β1, regulation

CLC Number:

R765.21

Jiao Jian, Zhang Luo. Effect of transforming growth factor-β1 on tight junction barrier function in nasal epithelial cells of chronic rhinosinusitis with nasal polyps[J]. Journal of Capital Medical University, 2018, 39(1): 45-50.

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Effect of transforming growth factor-β1 on tight junction barrier function in nasal epithelial cells of chronic rhinosinusitis with nasal polyps

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