Expression and glucocorticoid regulation of MALAT1 in chronic rhinosinusitis with nasal polyps

doi:10.3969/j.issn.1006-7795.2020.02.007

Abstract

Abstract: Objective To explore the expression of metastasis-associated lung adenocarcinoma transcript 1 (MALAT1) in different subtypes of chronic rhinosinusitis with nasal polyps (CRSwNP) and its response to glucocorticoid therapy. Methods Real-time PCR was used to detect the expression of MALAT1 in nasal tissues from eosinophilic CRSwNP, non-eosinophilic CRSwNP and healthy control subjects. The correlation between the expression of MALAT1 and Th1/Th2/Th17 cytokines was analyzed. Moreover, the effect of glucocorticoid treatment on MALAT1 expression was measured. Results MALAT1 was significantly down-regulated in both eosinophilic CRSwNP and non-eosinophilic CRSwNP compared to the healthy control. Moreover, the expression of MALAT1 was significantly lower in non-eosinophilic CRSwNP than that of eosinophilic CRSwNP. In contrast, interferon gamma (IFNG) was significantly up-regulated in both eosinophilic CRSwNP and non-eosinophilic CRSwNP compared to the healthy control. The Spearman correlation test showed that the expression of MALAT1 was negatively correlated with the expression of IFNG (r=-0.595,P=0.001). In addition, MALAT1 was significantly up-regulated by glucocorticoid treatment both in patients with eosinophilic CRSwNP and non-eosinophilic CRSwNP (P=0.015, respectively). Conclusion MALAT1 is significantly down-regulated in both eosinophilic CRSwNP and non-eosinophilic CRSwNP compared to the healthy control, and it negatively correlated with the expression of IFNG. Glucocorticoid treatment significantly up-regulate the expression of MALAT1 in CRSwNP.

Key words: chronic rhinosinusitis with nasal polyps, metastasis-associated lung adenocarcinoma transcript 1(MALAT1), interferon gamma, glucocorticoid

CLC Number:

R765

Wang Ming, Bu Xiangting, Luan Ge, Wang Yang, Wang Chengshuo, Zhang Luo. Expression and glucocorticoid regulation of MALAT1 in chronic rhinosinusitis with nasal polyps[J]. Journal of Capital Medical University, 2020, 41(2): 183-188.

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URL: https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/10.3969/j.issn.1006-7795.2020.02.007

https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/Y2020/V41/I2/183

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