Abstract:

Objective To explore whether there are differences between hemophagocytic syndrome patients and normal human bone marrow CD34⁺ cells' quality, study the effect and characteristics of interferon gamma differentiation to the bone marrow, and observe whether improved colony stimulating factor can restrain the inhibition of interferon gamma on bone marrow. Methods Separation and purification of bone marrow CD34⁺ hematopoietic stem cells were performed by immunomagnetic beads method. SCF, IL 3, GM-CSF, G-CSF, EPO colony stimulating factor on differentiation of bone marrow stem cells, the colony forming units (CFU) to evaluate the formation of bone marrow differentiation. Added different concentration gradient of interferon gamma in the system, observation the bone marrow colony formation the situation. Increase in the minimum concentrations interferon gamma inhibition of bone marrow in colony stimulating factor concentration to observe the bone marrow growth situation.Results This study demonstrated no difference in bone marrow CD34⁺ cells compared with normal quality (P> 0.05). The degree of inhibition of interferon gamma in different concentration on bone marrow is different, when interferon gamma concentration increased to more than 10ng/mL, the experimental group colony number difference compared to the control group without the addition of interferon gamma (P<0.05). To increase the training system of colony stimulating factors concentration can be made against the bone marrow suppression of interferon gamma.Conclusion There was no significant difference in bone marrow's quality between HPS patients and normal control. Interferon gamma may inhibit bone marrow's growth. Increased colony stimulating factor concentration may act against interferon gamma production.

Key words: bone marrow colony culture, hemophagocytic syndrome, interferon gamma, colony stimulating factor