miR-378a-5p down-regulates SphK1 expression in LX-2

doi:10.3969/j.issn.1006-7795.2019.01.017

Abstract

Abstract: Objective To investigate whether microRNA could regulate the expression of sphingosine kinase 1 (SphK1) mRNA in LX-2 cells. Methods Prediction of microRNAs was performed on bioinformatics sites. Real-time quantitative polymerase chain reaction (RT-qPCR) was applied to detect the expression of SphK1 mRNA in LX-2 cells. Results Only miR-378a-5p, not miR-92a-2-5p or miR-708-5p, could suppress the increase of SphK1 mRNA expression caused by transforming growth factor-β1 (TGF-β1) in LX-2. And the expression of SphK1 mRNA was up-regulated when the inhibitor of miR-378a-5p was applied in LX-2 cells. Conclusion miR-378a-5p took part in the regulation of SphK1 mRNA expression in LX-2, and it could down-regulate the increase of SphK1 mRNA expression caused by TGF-β1.

Key words: miR-378a-5p, sphingosine kinase 1 (SphK1), transforming growth factor-β1

CLC Number:

R329.2

Qi Changbo, Chang Na, Yang Lin, Li Liying. miR-378a-5p down-regulates SphK1 expression in LX-2[J]. Journal of Capital Medical University, 2019, 40(1): 94-100.

0
/ / Recommend

Add to citation manager EndNote|Reference Manager|ProCite|BibTeX|RefWorks

URL: https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/10.3969/j.issn.1006-7795.2019.01.017

https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/Y2019/V40/I1/94

References

[1] Friedman S L. Mechanisms of hepatic fibrogenesis[J]. Gastroenterology, 2008, 134(6):1655-1669.
[2] Cassiman D, Libbrecht L, Desmet V, et al. Hepatic stellate cell/myofibroblast subpopulations in fibrotic human and rat livers[J]. J Hepatol, 2002, 36(2):200-209.
[3] Liu X, Yue S, Li C, et al. Essential roles of sphingosine 1-phosphate receptor types 1 and 3 in human hepatic stellate cells motility and activation[J]. J Cell Physiol,2011, 226(9):2370-2377.
[4] Li C, Jiang X, Yang L, et al. Involvement of sphingosine 1-phosphate (SIP)/S1P3 signaling in cholestasis-induced liver fibrosis[J]. Am J Pathol, 2009, 175(4):1464-1472.
[5] Li C, Kong Y, Wang H, et al. Homing of bone marrow mesenchymal stem cells mediated by sphingosine 1-phosphate contributes to liver fibrosis[J]. J Hepatol, 2009, 50(6):1174-1183.
[6] Li C, Zheng S, You H, et al. Sphingosine 1-phosphate (S1P)/S1P receptors are involved in human liver fibrosis by action on hepatic myofibroblasts motility[J]. J Hepatol, 2011, 54(6):1205-1213.
[7] Woodhoo A, Iruarrizaga-Lejarreta M, Beraza N, et al. Human antigen R contributes to hepatic stellate cell activation and liver fibrosis[J]. Hepatology,2012, 56(5):1870-1882.
[8] Bataller R, Brenner D A. Liver fibrosis[J]. J Clin Invest, 2005, 115(2):209-218.
[9] Yang L, Chang N, Liu X, et al. Bone marrow-derived mesenchymal stem cells differentiate to hepatic myofibroblasts by transforming growth factor-beta1 via sphingosine kinase/sphingosine 1-phosphate (S1P)/S1P receptor axis[J]. Am J Pathol,2012, 181(1):85-97.
[10] Xiu L, Chang N, Yang L, et al. Intracellular sphingosine 1-phosphate contributes to collagen expression of hepatic myofibroblasts in human liver fibrosis independent of its receptors[J]. Am J Pathol, 2015, 185(2):387-398.
[11] Ge J, Chang N, Zhao Z, et al. Essential roles of RNA-binding protein HuR in activation of hepatic stellate cells induced by transforming growth factor-beta1[J]. Sci Rep, 2016, 6:22141.
[12] Vettori S, Gay S, Distler O. Role of MicroRNAs in Fibrosis[J]. Open Rheumatol J, 2012, 6:130-139.
[13] Jiang X, Tsitsiou E, Herrick S E, et al. MicroRNAs and the regulation of fibrosis[J]. FEBS J, 2010,277(9):2015-2021.
[14] Wang X W, Heegaard N H, Orum H. MicroRNAs in liver disease[J]. Gastroenterology, 2012, 142(7):1431-1443.
[15] Noetel A, Kwiecinski M, Elfimova N, et al. microRNA are central players in anti-and profibrotic gene regulation during liver fibrosis[J]. Front Physiol, 2012, 3:49.
[16] Li J, Wu H, Li W, et al. Downregulated miR-506 expression facilitates pancreatic cancer progression and chemoresistance via SPHK1/Akt/NF-kappaB signaling[J]. Oncogene, 2016,35(42):5501-5514.
[17] Zhao X, He W, Li J, et al. MiRNA-125b inhibits proliferation and migration by targeting SphK1 in bladder cancer[J]. Am J Transl Res, 2015, 7(11):2346-2354.
[18] Chen M B, Yang L, Lu P H, et al. MicroRNA-101 down-regulates sphingosine kinase 1 in colorectal cancer cells[J]. Biochem Biophys Res Commun, 2015, 463(4):954-960.
[19] Xia J, Wu Z, Yu C, et al. miR-124 inhibits cell proliferation in gastric cancer through down-regulation of SPHK1[J]. J Pathol, 2012, 227(4):470-480.
[20] Guo L, Zhang Y, Zhang L, et al. MicroRNAs, TGF-beta signaling, and the inflammatory microenvironment in cancer[J]. Tumour Biol, 2016, 37(1):115-125.
[21] Chen W, Zhou S, Mao L, et al. Crosstalk between TGF-beta signaling and miRNAs in breast cancer metastasis[J]. Tumour Biol, 2016,37(8):10011-10019.
[22] Berschneider B, Ellwanger D C, Baarsma H A, et al. miR-92a regulates TGF-beta1-induced WISP1 expression in pulmonary fibrosis[J]. Int J Biochem Cell Biol, 2014, 53:432-441.
[23] Liu Y, Saiyan S, Men T Y, et al. Hepatopoietin Cn reduces ethanol-induced hepatoxicity viasphingosine kinase 1 and sphingosine 1-phosphate receptors[J]. J Pathol,2013, 230(4):365-376.
[24] Gellings L N, Swaney J S, Moreno K M, et al. Sphingosine-1-phosphate and sphingosine kinase are critical for transforming growth factor-b-stimulated collagen production by cardiac fibroblasts[J].Cardiovasc Res,2009,82(2):303-312.
[25] Huang L S, Berdyshev E, Mathew B. Targeting sphingosine kinase 1 attenuates bleomycin-induced pulmonary fibrosis[J].FASEB J, 2013,27(4):1749-1760.
[26] Spiegel S, Milstien S. Functions of the multifaceted family of sphingosine kinases and some close relatives[J]. J Biol Chem,2007,282(4):2125-2129.
[27] Gellings L N, Swaney J S, Moreno K M, et al. Sphingosine-1-phosphate and sphingosine kinase are critical for transforming growth factor-beta-stimulated collagen production by cardiac fibroblasts[J]. Cardiovasc Res,2009, 82:303-312.
[28] Kono Y, Nishiuma T, Nishimura Y, et al. Sphingosine kinase 1 regulates differentiation of human and mouse lung fibroblasts mediated by TGF-β1[J]. Am J Respir Cell Mol Biol, 2007, 37(4):395-404.
[29] Ren S, Babelova A, Moreth K, et al. Transforming growth factor-beta2 upregulates sphingosine kinase-1 activity, which in turn attenuates the fibrotic response to TGF-beta2 by impeding CTGF expression[J]. Kidney Int,2009, 76(8):857-867.
[30] Cencetti F, Bernacchioni C, Nincheri P, et al. Transforming growth factor-beta1 induces transdifferentiation of myoblasts into myofibroblasts via up-regulation of sphingosine kinase-1/S1P3 axis[J]. Mol Biol Cell,2010, 21(6):1111-1124.
[31] Takuwa Y, Ikeda H, Okamoto Y, et al. Sphingosine-1-phosphate as a mediator involved in development of fibrotic diseases[J]. Biochim Biophysica Acta,2013, 1831(1):185-192.
[32] Hyun J, Wang S, Kim J, et al. MicroRNA-378 limits activation of hepatic stellate cells and liver fibrosis by suppressing Gli3 expression[J]. Nat Commun, 2016, 7:10993.
[33] Nagalingam R S, Sundaresan N R, Noor M, et al. Deficiency of cardiomyocyte-specific microRNA-378 contributes to the development of cardiac fibrosis involving a transforming growth factor beta (TGFbeta1)-dependent paracrine mechanism[J]. J Biol Chem, 2014, 289(39):27199-27214.
[34] 陈冉,王瑞.TGF-β1/MAPK信号转导通路与肺纤维化的关系[J].中国煤炭工业医学杂志,2016,19(9):1399-1403.
[35] 傅玉琼,周伟.转化生长因子β₁及成纤维细胞生长因子基因敲除对尘肺小鼠肺纤维化的影响[J].解放军医药杂志,2017,29(1):27-30.