Senegenin promotes in vitro proliferation and differentiation of human neural progenitor cells

doi:10.3969/j.issn.1006-7795.2013.04.016

Abstract

Abstract:

Objective To study the effects of senegenin on the proliferation and differentiation of human neural stem cells in vitro and its possible mechanism.Methods Human neural stem cell line was cultured and identified in vitro. They were incubated with 50 μmol/L or 5 μmol/L senegenin respectively, and the group added with the vehicle was set as the control. Then the cell absorbance was measured by CCK8 assay on a microplate reader to test the cell viability. The effects of 50 μmol/L or 5 μmol/L senegenin on the mRNA expression of negative differentiation regulating gene Hes1 and positive differentiation regulating gene Mash1 were examined by real-time fluorescence quantitative RT-PCR. The differentiation ratio of neurons and astrocytes from the neural stem cells was assessed through immune-fluorescence staining process.Results Senegenin at 50 μmol/L significantly increased the cell number compared with the control group(P<0.05), and upregulated the Hes1 mRNA expression(P<0.05). Senegenin at 5 μmol/L also promoted the cell proliferation, and at the same time it increased the mRNA expressions of both Hes1 and Mash1 apparently(P<0.05). Moreover, 5 μmol/L senegenin obviously promoted the neuronal differentiation from the neural stem cells. Conclusion Senegenin can promote the proliferation and differentiation of human neural stem cells in vitro, and this effect may be induced by the up-regulation of Hes1 and Mash1 gene expression.

Key words: senegenin, human neural stem cells, proliferation, differentiation

CLC Number:

R338

SHI Fang, LIANG Zhigang, GUO Zixuan, WANG Xuan, WANG Xiaomin. Senegenin promotes in vitro proliferation and differentiation of human neural progenitor cells[J]. Journal of Capital Medical University, 2013, 34(4): 559-565.

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URL: https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/10.3969/j.issn.1006-7795.2013.04.016

https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/Y2013/V34/I4/559

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