Establishment a mesenchymal stem cell line model derived from Klinefelter syndrome fetal umbilical cord

doi:10.3969/j.issn.1006-7795.2019.06.012

Journal of Capital Medical University ›› 2019, Vol. 40 ›› Issue (6): 868-874.doi: 10.3969/j.issn.1006-7795.2019.06.012

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Establishment a mesenchymal stem cell line model derived from Klinefelter syndrome fetal umbilical cord

Luo Jianan, Yang Shufa, Yu Xiuyi, Liu Xin, Chen Zhenwen

Department of Medical Genetics and Developmental Biology, School of Basic Medical Sciences, Capital Medical University, Beijing 100069, China

Received:2019-04-23 Online:2019-11-21 Published:2019-12-18
Supported by:
This study was supported by National Natural Science Foundation of China (31772545),Science and Technology Plan of Beijing Education Commission (KM201910025027).

Abstract

Abstract: Objective We succeeded in establishing a mesenchymal stem cell line (Klinefelter's syndrome umbilical cord mesenchymal stem cells, KUMSCs) from Klinefelter syndrome (KS) fetal umbilical cord for the klinefelter's syndrome disease research. Methods Umbilical cords of aborted fetuses with Klinefelter syndrome were obtained from the clinical hospital. KUMSCs were isolated and expanded in vitro, and the analytical techniques included:quality detection, flow cytometry, karyotype analysis and cell differentiation experiments. Results The content of pathogens (bacteria, fungi, viruses, spirochetes, mycoplasma) and endotoxin of mesenchymal stem cells were all in line with the quality control standards, KUMSCs cells were positive for CD73, CD90, CD29, CD105 and CD44, negative for CD34, CD45 and human leukocyte antigen-DR(HLA-DR). Cell differentiation assay results indicate that KUMSCs can be induced to differentiate into adipocytes, osteoblasts. Conclusion These results indicate that a model of umbilical cord mesenchymal stem cells derived from KS has been successfully established, which can be applied to the experimental study of the pathogenesis and drug development of Klinefelter syndrome.

Key words: Klinefelter syndrome, umbilical cord mesenchymal stem cells, osteogenic differentiation, adipogenic differentiation

CLC Number:

R394

Luo Jianan, Yang Shufa, Yu Xiuyi, Liu Xin, Chen Zhenwen. Establishment a mesenchymal stem cell line model derived from Klinefelter syndrome fetal umbilical cord[J]. Journal of Capital Medical University, 2019, 40(6): 868-874.

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Establishment a mesenchymal stem cell line model derived from Klinefelter syndrome fetal umbilical cord

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