Therapeutic effect of hypoxia-preconditioned human umbilical cord mesenchymal stem cell on premature ovarian insufficiency mouse model

doi:10.3969/j.issn.1006-7795.2019.04.014

Journal of Capital Medical University ›› 2019, Vol. 40 ›› Issue (4): 566-571.doi: 10.3969/j.issn.1006-7795.2019.04.014

• Gynecological Endocrinology and Menopause • Previous Articles Next Articles

Therapeutic effect of hypoxia-preconditioned human umbilical cord mesenchymal stem cell on premature ovarian insufficiency mouse model

Zhao Yue¹, Ruan Xiangyan^1,2, Wang Fengchun³, Li Yanglu¹, Cheng Jiaojiao¹, Alfred O. Mueck^1,2

1. Department of Endocrinology, Beijing Obstetrics and Gynecology Hospital, Capital Medical University, Beijing 100026, China;
2. Department for Women's Health, University Women's Hospital and Research Center for Women's Health, University of Tuebingen, Tuebingen D-72076, Germany;
3. Department of Delivery, Beijing Obstetrics and Gynecology Hospital, Capital Medical University, Beijing 100026, China

Received:2019-05-20 Online:2019-07-21 Published:2019-07-19
Supported by:
This study was supported by Beijing Municipal Administration of Hospital' Youth Programme (QML20181401), Capital Medical University Research and Development Fund (PYZ2018161),Beijing Obstetrics and Gynecology Hospital, Capital Medical University (FCYY201811),Beijing Municipal Administration of Hospitals Clinical Medicine Development of Special Funding Support(XMLX201710), Beijing Municipal Science and Technology Commission (Z161100000516143).

Abstract

Abstract: Objective To investigate the therapeutic effect of hypoxia-preconditioned human umbilical cord mesenchymal stem cells (hUCMSCs) on premature ovarian insufficiency (POI) mice model. Methods hUCMSCs were isolated and cultured from umbilical cord, and POI mice model were induced by busulfan and cyclophosphamide. POI mice were treated with normal hUCMSCs and hypoxic pretreated hUCMSCs. At the end of the experiment, the levels of estradiol (E2), follicle stimulating hormone (FSH) and anti Mullerian hormone (AMH) in the plasma were measured, histopathological examination was performed on ovaries, and the number of follicles was counted. Results Plasma E2 and AMH levels in mice treated with hUCMSCs and hypoxic preconditioned hUCMSCs showed an increasing trend, with a significantly different in AMH levels (P<0.05) compared with the control group. The growth of follicles in the ovarian tissue of the hUCMSCs and hypoxic preconditioned hUCMSCs groups was significantly increased, and the atresia follicles were significantly decreased (P<0.05). The color of the ovary was ruddy, and the size was increased, with almost no necrosis. The follicles were large, with good transparency. The ovarian status of the normal control group was similar, and the number of growing follicles and atresia follicles in the treated groups became more normal. Conclusion hUCMSCs transplantation significantly improved the serum levels of high gonadotropin and low estrogen of POI mice, and promote follicular development.

Key words: human umbilical cord mesenchymal stem cells, premature ovarian insufficiency, premature ovarian failure, animal experiment

CLC Number:

R711

Zhao Yue, Ruan Xiangyan, Wang Fengchun, Li Yanglu, Cheng Jiaojiao, Alfred O. Mueck. Therapeutic effect of hypoxia-preconditioned human umbilical cord mesenchymal stem cell on premature ovarian insufficiency mouse model[J]. Journal of Capital Medical University, 2019, 40(4): 566-571.

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Therapeutic effect of hypoxia-preconditioned human umbilical cord mesenchymal stem cell on premature ovarian insufficiency mouse model

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