The effects and mechanism of phenylethyl isothiocyanate on cell proliferation and apoptosis in BPH-1 cells

doi:10.3969/j.issn.1006-7795.2021.06.010

Abstract

Abstract: Objective To investigate the effects of phenethyl isothiocyanate (PEITC) on human benign prostatic epithelial cells(BPH-1). Methods Exposure of BPH-1 cells to PEITC at different concentrations (6, 12, and 24 μmol/L) for 6, 12, 24, or 36 hours. The suppression of cell proliferation was assessed with CCK-8 assay. The cell apoptosis was evaluated with Annexin V/PI staining by fluorescent flow-cytometric assay. The Western blotting analysis was applied to examine the expression of X-linked inhibitor-of-apoptosis protein (XIAP) and Atg5-12. The expression of XIAP mRNA was detected with RT-PCR. Results 1)Upon the treatment of PEITC at 6, 12, and 24 μmol/L, the cell relative viability was 71.22%, 48.90%, and 13.57%, respectively, for 6 hours; 52.57%, 42.37%, and 10.11%, respectively, for 12 hours; 36.73%, 25.88%, and 8.39% for 24 hours, respectively, and 33.57%, 25.83%, and 13.06%, respectively, for 36 hours. PEITC suppressed the proliferation of BPH-1 cells in a concentration-dependent way, compared with the control group (P<0.05). 2)The total percentages of apoptotic cells was 19.5%, 30.4%, and 40.1% in BPH-1 cells upon the treatment of PEITC at 6, 12, and 24 μmol/L for 24 hours, respectively. PEITC promoted cell apoptosis at concentrations of 12 μmol/L and 24 μmol/L, compared with the control group (P<0.05). 3)The relative expression of XIAP mRNA and XIAP was suppressed in a concentration-dependent way (6, 12, and 24 μmol/L) upon the treatment of PEITC for 24 hours. The expression of Atg 5-12 protein was also inhibited in a concentration-dependent way (6, 12, and 24 μmol/L) upon the treatment of PEITC for 24 hours. Conclusions PEITC suppressed the proliferation of benign prostatic epithelial cells and induced cell apoptosis by inhibiting the expression of XIAP mRNA and XIAP in a concentration-dependent way, which was probably associated with the suppressed autophagy pathway in BPH-1 cells.

Key words: phenethyl isothiocyanate(PEITC), proliferation, apoptosis, autophagy, X-linked inhibitor of apoptosis(XIAP), Atg5-12

CLC Number:

R697⁺.3

Liu Zhibin, Jin Song, Niu Yinong. The effects and mechanism of phenylethyl isothiocyanate on cell proliferation and apoptosis in BPH-1 cells[J]. Journal of Capital Medical University, 2021, 42(6): 961-966.

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URL: https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/10.3969/j.issn.1006-7795.2021.06.010

https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/Y2021/V42/I6/961

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