Effects of baicalin on cognitive function and expression of LC-3Ⅱ and Beclin-1 proteins in hippocampus of rats with chronic cerebral hypoperfusion

doi:10.3969/j.issn.1006-7795.2019.03.002

Abstract

Abstract: Objective To investigate the effects of baicalin on the cognitive ability and the expression of LC-3 Ⅱ and Beclin-1 proteins in hippocampus of the rats with chronic cerebral hypoperfusion. Methods A total of 50 rats were randomly divided into five groups:control group, model group, low,medium, and high dose of baicalin groups with 10 rats in each group. The chronic cerebral hypoperfusion rat model was established by permanent bilateral common carotid artery occlusion.Baicalin groups were treated with intraperitoneal injection of baicalin in three different dosages (60,120,240 mg·kg^-1·d^-1), while those in the other two groups were treated with the same volume of saline. After four weeks, the spatial learning and memory ability were observed by Morris water maze test, and the expression of LC-3 Ⅱ and Beclin-1 in the hippocampus were revealed with Western blotting.Results Both escape latency and swimming distance of the chronic cerebral hypoperfusion rats were significantly prolonged in comparison of those in normal control (P<0.01), and swimming time in the platform of previous quadrant was significantly shorter in model group (P<0.01). Furthermore, the expression of LC-3 Ⅱ and Beclin-1 protein increased in model group compared with the control group (P<0.01). Four weeks of treatment with baicalin remarkably improved the learning and memory ability of chronic cerebral hypoperfusion rats (P<0.01 or P<0.05), decreased the expression of LC-3 Ⅱ and Beclin-1 in hippocampus of the model rats (P<0.01 or P<0.05). Conclusion Baicalin can improve cognitive ability of the chronic cerebral hypoperfusion rats, and inhibit autophagy by decreasing the expression of LC-3 Ⅱ and Beclin-1 in the hippocampus.

Key words: baicalin, chronic cerebral hypoperfusion, cognitive ability, autophagy

CLC Number:

R255.4

Chen Yujing, Chen Wenqiang, Zhang Jianxin, Liu Yan, Wu Xiling, Wang Jiandong, Huang Xiaobo. Effects of baicalin on cognitive function and expression of LC-3Ⅱ and Beclin-1 proteins in hippocampus of rats with chronic cerebral hypoperfusion[J]. Journal of Capital Medical University, 2019, 40(3): 330-334.

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URL: https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/10.3969/j.issn.1006-7795.2019.03.002

https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/Y2019/V40/I3/330

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