Effects of Jianpi Liqi formula on MLCK and PGE2-cAMP signal path in the duodenum of rats with functional dyspepsia

doi:10.3969/j.issn.1006-7795.2019.03.003

Journal of Capital Medical University ›› 2019, Vol. 40 ›› Issue (3): 335-339.doi: 10.3969/j.issn.1006-7795.2019.03.003

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Effects of Jianpi Liqi formula on MLCK and PGE2-cAMP signal path in the duodenum of rats with functional dyspepsia

Zhao Luqing¹, Chang Xiongfei², Zhang Shengsheng¹

1. Digestive Disease Center, Beijing Hospital of Traditional Chinese Medicine, Capital Medical University, Beijing 100010, China;
2. Gastroenterology Department, Beijing Shunyi Hospital of Traditional Chinese Medicine, Beijing 101300, China

Received:2019-03-15 Online:2019-05-21 Published:2019-06-13
Supported by:
This study was supported by National Natural Science Foundation of China(81774215,81503514), Beijing Municipal Administration of Hospitals' Youth Program(QML20161002), Xu-Xia Fund for Young Scholars(XX-201703).

Abstract

Abstract: Objective To explore the effect of Jianpi Liqi formula(JPLQ) on myosin light chain kinase(MLCK) and prostaglandin E2-cyclic adenosine monophosphate(PGE2-cAMP) signaling pathway in the duodenum of functional dyspepsia(FD) rats. Methods Eighteen SD male rats were randomly divided into control group,FD model group and JPLQ group in a 1:1:1 ratio. Rats with FD were generated via stress-based tail clamping. Stressed rats were given JPLQ or saline in the drinking water, and the morphology of duodenum was measured by hematoxylin-eosin(HE) staining, mRNA expression of MLCK and PGE2 receptor subtype 1 and 4(EP1,EP4) were analyzed with quantitative real time polymerase chain reaction (qRT-PCR). PGE2 and cAMP expression was measured with enzyme-linked immunosorbent assay (ELISA).Results MLCK mRNA expression in duodenum was decreased in the JPLQ-treated stressed rats as compared to the control-treated stressed rats. PGE2, EP4 and cAMP in duodenum was increased in the JPLQ-treated stressed rats as compared to the control-treated stressed rats. There was no difference of EP1 expression among the three groups. Conclusion The underlying mechanisms of JPLQ formula on FD are at least partially through the attenuation of MLCK signaling pathway and the improvement of PGE2-EP4-cAMP signaling pathway.

Key words: Jianpi Liqi formula, functional dyspepsia, duodenum, myosin light chain kinase, prostaglandin E2

CLC Number:

R256.3

Zhao Luqing, Chang Xiongfei, Zhang Shengsheng. Effects of Jianpi Liqi formula on MLCK and PGE2-cAMP signal path in the duodenum of rats with functional dyspepsia[J]. Journal of Capital Medical University, 2019, 40(3): 335-339.

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URL: https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/10.3969/j.issn.1006-7795.2019.03.003

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Effects of Jianpi Liqi formula on MLCK and PGE2-cAMP signal path in the duodenum of rats with functional dyspepsia

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