Alterations in the expression of β-adrenoceptor subtypes in duodenal mucosa of Parkinson’s rats

doi:10.3969/j.issn.1006-7795.2014.04.016

Journal of Capital Medical University ›› 2014, Vol. 35 ›› Issue (4): 463-467.doi: 10.3969/j.issn.1006-7795.2014.04.016

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Alterations in the expression of β-adrenoceptor subtypes in duodenal mucosa of Parkinson’s rats

Feng Xiaoyan, Fan Ruifang, Sun Lu, Zhang Yue, Fu Xiaosuo, Zhu Jinxia

Department of Physiology and Pathophysiology, School of Basic Medical Sciences, Capital Medical University, Beijing 100069, China

Received:2014-01-09 Online:2014-08-21 Published:2014-07-22
Supported by:
This study was supported by National Natural Science Foundation of China (81170346), Beijing Natural Science Foundation(7121003, 7132017), Basic and Clinical Research Cooperation Fund of Capital Medical University.

Abstract

Abstract:

Objective To investigate the alterations of β-adrenoceptor subtypes in the duodenal mucosa of 6-hydroxydopamine(6-OHDA)-induced Parkinson's disease(PD) rat models. Methods Rats were subjected with 6-OHDA in bilateral substantia nigra(SN) to make the PD rat models. The location and quantitative detecting techniques including immunohistochemistry and real-time PCR were used to determine the alterations of β-adrenoceptor subtypes(β₁, β₂, β₃) in the PD rats. Results All three subtypes of β-adrenoceptor were found to express in the epithelial layer of duodenum in the model and control rats. However, the levels of the three receptors were higher in model rats than those of control rats with the β₂ subtype being most prominent. Conclusion The β-adrenoceptors(β₁, β₂, β₃) are up-regulated in the duodenal mucosa of 6-OHDA-induced PD rats, suggesting a potential mechanism for the gastrointestinal dysfunction in PD.

Key words: 6-hydroxydopamine(6-OHDA)-induced Parkinson’s disease rats, duodenum, β-adrenoceptor

CLC Number:

R592

Feng Xiaoyan, Fan Ruifang, Sun Lu, Zhang Yue, Fu Xiaosuo, Zhu Jinxia. Alterations in the expression of β-adrenoceptor subtypes in duodenal mucosa of Parkinson’s rats[J]. Journal of Capital Medical University, 2014, 35(4): 463-467.

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Alterations in the expression of β-adrenoceptor subtypes in duodenal mucosa of Parkinson’s rats

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