Establishment of hepatic metastasis model of colorectal cancer for optical in vivo imaging in mice with bioluminescence and near-infrared luminescence

doi:10.3969/j.issn.1006-7795.2014.04.017

Abstract

Abstract:

Objective To detect hepatic metastasis and tumor angiogenesis in vivo imaging with bioluminescence and fluorescence in hepatic metastasis model of colorectal cancer. Methods Mice were inoculated with SL4 cells, colorectal cancer cell line, stably expressed firefly luciferase gene, after 2 weeks of the injection we detect hepatic metastases in vivo imaging in mice with bioluminescence and also determine tumor angiogenesis with in vivo imaging in mice with fluorescence. Results We confirmed hepatic metastasis over time with luciferase activity, and also found tumor angiogenesis in hepatic metastasis foci, which confirmed by immunostaining of CD34 marker of vessels.Conclusion Establishment of hepatic metastasis model of colorectal cancer for optical in vivo imaging in mice with bioluminescence and fluorescence.

Key words: optical in vivo imaging, bioluminescence, near-infrared luminescence, colorectal cancer, hepatic metastases

CLC Number:

R735.3

Piao Chunmei, Liu Xuxia, Wang Lvya, Du Jie. Establishment of hepatic metastasis model of colorectal cancer for optical in vivo imaging in mice with bioluminescence and near-infrared luminescence[J]. Journal of Capital Medical University, 2014, 35(4): 468-472.

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URL: https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/10.3969/j.issn.1006-7795.2014.04.017

https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/Y2014/V35/I4/468

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