Journal of Capital Medical University ›› 2014, Vol. 35 ›› Issue (4): 473-476.doi: 10.3969/j.issn.1006-7795.2014.04.018

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Tumor suppressor gene Rb1 was down-regulated by miR-26a in Y79 retinoblastoma cells

Bai Jing1, Chu Dong2, Gao Hai3, Liu Xiangyi1   

  1. 1. Department of Clinical Laboratory, Beijing Tongren Hospital, Capital Medical University, Beijing 100730, China;
    2. Department of Orthopedic, Air Force General Hospital, Beijing 100142, China;
    3. Emergency Center, Beijing Anzhen Hospital, Capital Medical University, Beijing 100029, China
  • Received:2014-04-24 Online:2014-08-21 Published:2014-07-22
  • Supported by:

    This study was supported by National Natural Science Foundation of China(81001044), Base and Clinical Fund of Capital Medical University(14JL60).

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Abstract:

Objective To explore the mechanism on down-regulation ofRb1expression in Rb cells. Methods In our study, the expression of miR-26a andRb1was detected by real-time PCR and Western blotting, respectively. Results Here, it was shown that miR-26a was differentially expressed in human retinoblastoma cells and tissues, compared with human retinal microvascular endothelial cells. Our results also showed that miR-26a tightly regulated the expression ofRb1by specifically targeting a conserved sequence motif in its 3' UTR, resulting in a low expression ofRb1in retinoblastoma cells. Conclusion The expression of tumor supressor geneRb1was down-regulated by miR-26a in Y79 Rb cells.

Key words: retinoblastoma, miR-26a, tumor supressor gene

CLC Number: