Epimedium flavonoids improve white matter lesions induced by chronic cerebral hypoperfusion in rats

doi:10.3969/j.issn.1006-7795.2021.05.012

Abstract

Abstract: Objective To investigate the effects of epimedium flavonoids (EF) on white matter lesions induced by chronic cerebral hypoperfusion in rats. Methods Male SD rats were subjected to permanent bilateral common carotid artery ligation (2VO) surgery. All rats were randomly divided into 5 groups: sham group, model group, EF 100 mg/kg group, EF 200 mg/kg group and nimodipine group. Drugs were administered intragastrically at 24 h after surgery for 6 weeks. The new object recognition test was used to assess the spatial memory recognition ability of rats. Diffusion tensor imaging was used to detect the changes of microstructure and fractional anisotropy coefficient (FA), apparent diffusion coefficient (ADC), axial diffusion coefficient (AD) and radial diffusivity coefficient (RD) in corpus callosum and optic tract of white matter in 2VO model rats. White matter structure was also observed with luxol fast blue (LFB) staining. Results After 2VO surgery the discrimination index in the new object recognition test significantly decreased, the value of FA decreased, and the RD value increased, indicating the damage of white matter in 2VO rats. In addition, the grading score of LFB staining was significantly downregulated. While treatment with EF increased the discrimination index, reduced the microstructure damage of corpus callosum and optic tract, protected the integrity of nerve fibers, decreased the grading score of LFB staining in corpus callosum of 2VO model rats, alleviated the demyelination of myelin. Conclusion EF treatment could reduce the structural abnormalities of the myelin sheath in rats after 2VO operation, alleviate the lesion of white matter, and improve the learning and memory dysfunction in 2VO rats.

Key words: epimedium flavonoids, chronic cerebral hypoperfusion, white matter lesion, corpus callosum, myelin sheath injury

CLC Number:

R745.1

Zhang Li, Wang Mingyang, Niu Hongmei, Zhang Lan, Li Lin. Epimedium flavonoids improve white matter lesions induced by chronic cerebral hypoperfusion in rats[J]. Journal of Capital Medical University, 2021, 42(5): 768-775.

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URL: https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/10.3969/j.issn.1006-7795.2021.05.012

https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/Y2021/V42/I5/768

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