Effects of Yuanzhi decoction on cognitive function and apoptosis-related protein in hippocampus of rats with chronic cerebral hypoperfusion

doi:10.3969/j.issn.1006-7795.2019.03.001

Abstract

Abstract: Objective To observe the effects of Yuanzhi decoction on spatial learning and memory ability and apoptosis-related protein in the hippocampus of rats with chronic cerebral hypoperfusion. Methods A rat model was developed with with ligating the bilateral common carotid arteries permanently.A total of 42 SD rats were divided into 7 groups according to the digital random method:control, model, sham, Yuanzhi decoction high[20 g/(kg·d)], medium[10 g/(kg·d)]and low[5 g/(kg·d)] dose intervention group, and the positive drug control group[donepezil hydrochloride 0.52 mg/(kg·d)] (n=6 in each group). The rats were treated with intragastric administration once a day for 4 weeks. The Morris water maze positioning navigation experiment and space exploration experiment were used to evaluate the spatial learning and memory ability in rats. Immunofluorescence staining was used to examine the expression of apoptosis-related proteins B-cell lymphoma-2 (Bcl-2), Bcl-2 associated X protein (Bax) and cysteinyl aspartate specific proteinase-3 (Caspase-3) in the hippocampus of rats.Results Compared with the control group and the sham group, the escape latency and search distance of the model group were significantly longer (P<0.01), and the search time in the quadrant of the original platform was significantly shorter (P<0.01). The high, medium and low dose of Yuanzhi decoction intervention can significantly shorten the escape latency and search distance (P<0.01), and prolong the search time in the quadrant of the original platform (P<0.01). The expression of Bax and Caspase-3 in the hippocampus of the model group were significantly increased compared with the control group and the sham group (P<0.05 or P<0.01), and Bcl-2 was significantly decreased (P<0.01). The intervention of high and medium dose of Yuanzhi decoction can significantly reduce the expression of Bax and Caspase-3 (P<0.05) and increase the expression of Bcl-2 (P<0.05 or P<0.01). Conclusion Yuanzhi decoction could regulate the expression of apoptosis-related proteins Bcl-2, Bax and Caspase-3 in hippocampus and improve cognitive function of rats with chronic cerebral hypoperfusion.

Key words: Yuanzhi decoction, cognitive impairment, apoptosis, chronic cerebral hypoperfusion, hippocampus

CLC Number:

R243

Liu Yan, Chen Yujing, Chen Wenqiang, Huang Xiaobo. Effects of Yuanzhi decoction on cognitive function and apoptosis-related protein in hippocampus of rats with chronic cerebral hypoperfusion[J]. Journal of Capital Medical University, 2019, 40(3): 323-329.

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URL: https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/10.3969/j.issn.1006-7795.2019.03.001

https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/Y2019/V40/I3/323

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