Change of autophagy in the isoprenaline-induced cardiac dysfunction

doi:10.3969/j.issn.1006-7795.2016.06.001

Abstract

Abstract: Objective To observe the change of myocardial autophagy during the isoprenaline-induced mice cardiac dysfunction. Methods The mice model of cardiac dysfunction was established by intraperitoneal injection of isoprenaline persistently for 4 weeks. Cardiac function was monitored by ultrasonic imaging system; hemodynamic changes were measured via tail-cuff method; autophagy-related protein expressions of LC3Ⅱ/LC3Ⅰand p62 were detected by Western blotting. Results After treatment with isoprenaline for 4 weeks, ejection fraction and fractional short reduced, interventricular septum increased, the ratio of early peak flow velocity and atrium peak flow velocity (E/A) tended to be elevated, suggesting declined cardiac function in model group compared with control group. Correspondingly, the heart rate and diastolic blood pressure decreased in model group compared with control group. After the injection of isoprenaline for 1 day and 3 days, the level of LC3Ⅱ/LC3Ⅰincreased and relative expression of p62 increased, but both returned to normal levels at the 4th week. Conclusion In the progress of isoprenaline-induced cardiac dysfunction, myocardial autophagy increased at an early stage, then returned to the normal level appearing to be a dynamic trend.

Key words: isoprenaline, cardiac dysfunction, autophagy

CLC Number:

R541

Bai Yan, Xu Wenli, Lyu Tingting, Wu Ye, Wang Li, Wang Wen, Ma Xinliang, Liu Huirong. Change of autophagy in the isoprenaline-induced cardiac dysfunction[J]. Journal of Capital Medical University, 2016, 37(6): 719-724.

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URL: https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/10.3969/j.issn.1006-7795.2016.06.001

https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/Y2016/V37/I6/719

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