Autophagy is involved in DJ-1 protecting against rotenone-induced dopaminergic neurons injuries of substantia nigra in rats

doi:10.3969/j.issn.1006-7795.2013.03.013

Abstract

Abstract:

Objective To observe whether DJ-1 overexpression could attenuate rotenone-induced dopaminergic neurons injuries in substantia nigra (SN) of rats and its mechanism. Methods AAV vectors encoding human wild-type DJ-1, LacZ or GFP-DJ-1, GFP genes were stereotactically injected into the rat SN 4 weeks prior to rotenone lesion in SN. As identification of DJ-1 gene expression by immunohistochemistry, the numbers of TH positive neurons were detected to verify the neuron-protective effect of DJ-1. Autophagy-related proteins expression were investigated via Western blotting. Results After 4 weeks rotenone injected, the numbers of TH positive neurons increased significantly in impaired SN of rats in DJ-1 overexpressing group compared to rotenone-injected rats (P<0.05). Autophagy-related protein Beclin1, p-mTOR expression and LC3Ⅱ/Ⅰ ratio were detected in impaired SN of animals according to Western blotting. In DJ-1 overexpression group, Beclin 1 expression and the ratio of LC3Ⅱ to LC3I increased significantly compared with control groups, while p-mTOR protein expression decreased significantly compared with control groups (P<0.05).Conclusion DJ-1 could attenuate dopaminergic cells injuries induced by rotenone and upregulated autophagy level.

Key words: DJ-1, rotenone, autophagy, rat

CLC Number:

R 742.5

LIU Huifeng, YANG Weiwei, GAO Hua, YANG Hui. Autophagy is involved in DJ-1 protecting against rotenone-induced dopaminergic neurons injuries of substantia nigra in rats[J]. Journal of Capital Medical University, 2013, 34(3): 385-390.

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URL: https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/10.3969/j.issn.1006-7795.2013.03.013

https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/Y2013/V34/I3/385

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