miR-29s inhibit ACEA-induced J774A.1 cell migration by down regulation CB1 expression

doi:10.3969/j.issn.1006-7795.2018.02.015

Abstract

Abstract: Objective To investigate whether miR-29a-3p, miR-29b-3p and miR-29c-3p affect cannabinoid receptor 1(CB1)expression and cell migration in J774A.1 cells. Methods RT-qPCR was used to detect CB1 mRNA expression in J774A.1 cells. Boyden chamber assay was used for the detection of cell migration. Results In this study, we presented evidences that CB1 agonist Arachidonyl-2'-chloroethylamide (ACEA) upregulates the expression of CB1 mRNA in J774A.1 cells. Whereas miR-29a-3p or miR-29b-3p or miR-29c-3p mimics blocks the increase of CB1 mRNA level and ACEA-induced cell migration in J774A.1 cells. Conclusion miR-29s inhibit ACEA-induced J774A.1 cell migration via downregulating CB1 expression.

Key words: miR-29, cannabinoid receptor type 1 (CB1), cell migration

CLC Number:

Duan Xianghui, Chang Na, Li Liying. miR-29s inhibit ACEA-induced J774A.1 cell migration by down regulation CB1 expression[J]. Journal of Capital Medical University, 2018, 39(2): 230-235.

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URL: https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/10.3969/j.issn.1006-7795.2018.02.015

https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/Y2018/V39/I2/230

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