Effects of angiotensin converting enzyme 2 on apoptosis of hepatocytes

doi:10.3969/j.issn.1006-7795.2018.02.014

Abstract

Abstract: Objective To identify the role of angiotensin-converting enzyme 2 (ACE2) in apoptosis of hepatic cells and to characterize the related mechanisms. Methods 1 HepG2 cells were transfected with ACE2 over expression plasmid DNA; 2MTT assay was used to measure HepG2 cells viability which over expressed ACE2 gene and then exposed to palmitic acid; 3 TUNEL assay was used to detect the apoptosis of hepatocytes in age-matched male C57BL/6 and ACE2^-/y mice; 4The expression of apoptosis related genes were detected by RT-PCR; 5Western blotting was used to study the expression of Bcl2, Bax, CHOP, phosphorylation-JNK and caspase3. Results 1We determined that exposure to different concentration of PA for 24 h caused a decrease in cell viability. However, the cell viability in ACE2-overexpressing HepG2 cells was significantly higher than GFP group (when the concentration of PA was 0.4, 0.8 or 1.0 mmol/L, P<0.05). 2The TUNEL positive hepatocytes in age-matched male ACE2^-/y mice group were more than those of C57BL-6 mice in the control group. 3In ACE2-overexpressing HepG2 cells, the relative protein expressions of Bax, caspase-3, CHOP and phosphorylation-JNK were decreased. While, the expression of Bcl2 was increased significantly. At this stage, over-expression of ACE2 also down-regulated the expression of ER-stress related apoptosis pathway genes. Conclusion ACE2 could be involved in anti-apoptotic via resistance to endoplasmic reticulum stress.

Key words: angiotensin-converting enzyme 2, endoplasmic reticulum stress, apoptosis

CLC Number:

R587.1

Song Lini, Cao Xi, Liu Wei, Yang Jinkui. Effects of angiotensin converting enzyme 2 on apoptosis of hepatocytes[J]. Journal of Capital Medical University, 2018, 39(2): 223-229.

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URL: https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/10.3969/j.issn.1006-7795.2018.02.014

https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/Y2018/V39/I2/223

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