Clinical importance of ACE2 expression in colon cancer and susceptibility of colon cancer cells to three human coronaviruses using ACE2 as their receptor

doi:10.3969/j.issn.1006-7795.2023.04.024

Abstract

Abstract: Objective To investigate the expression of angiotensin-converting enzyme 2 (ACE2) in colon cancer and the relationship with prognosis as well as the susceptibility of colon cancer cells to three human coronaviruses who use ACE2 as their receptors. Methods Quantitative real-time Polymerase Chain Reaction, immunohistochemistry and Western blotting were used to explore the expression of ACE2.Databases were used to analyze the relationship between ACE2 expression and the prognosis of colon cancer patients. The susceptibility of colon cancer cells to three human coronaviruses who use ACE2 as receptors was analyzed based on pseudovirions. Results The expression levels of ACE2 in colon cancer were higher and the expression level was not correlated with prognosis. The susceptibility of Caco2 to severe acute respiratory syndrome coronavirus (SARS-CoV) pseudovirions was higher than that in CCC-HIE-2. Compared with CCC-HIE-2, the susceptibility of colon cancer cells to SARS-CoV-2 was higher, except for that in HCT-15. The susceptibility of all colon cancer cells to human coronaviruses NL63 (HCOV-NL63) was higher than that in CCC-HIE-2. Conclusions The expression level of ACE2 was increased in colon cancer and its expression was not correlated with prognosis. And the susceptibility of most colon cancer cells to three human coronaviruses was increased.

Key words: colon cancer, angiotensin-converting enzyme 2, human coronavirus, susceptibility

CLC Number:

R735

Ning Tingting, Wang Zhaoqing, Zhang Nan, Zhu Shengtao. Clinical importance of ACE2 expression in colon cancer and susceptibility of colon cancer cells to three human coronaviruses using ACE2 as their receptor[J]. Journal of Capital Medical University, 2023, 44(4): 663-668.

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URL: https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/10.3969/j.issn.1006-7795.2023.04.024

https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/Y2023/V44/I4/663

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