Mouse monocyte/macrophage cell line J774A.1 migration induced by the activation of CB1 depends on HuR

doi:10.3969/j.issn.1006-7795.2016.01.015

Journal of Capital Medical University ›› 2016, Vol. 37 ›› Issue (1): 76-82.doi: 10.3969/j.issn.1006-7795.2016.01.015

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Mouse monocyte/macrophage cell line J774A.1 migration induced by the activation of CB1 depends on HuR

Zhao Zhongxin, Chang Na, Ge Jingjing, Tian Lei, Li Liying

Department of Cell Biology, School of Basic Medical Sciences, Municipal Laboratory for Liver Protection and Regulation of Regeneration, Capital Medical University, Beijing 100069, China

Received:2015-07-09 Online:2016-02-21 Published:2016-02-01
Supported by:
This study is supported by TCM Preventive Medicine Major Cultivate Discipline Construction Project of National Traditional Chinese Medicine[(2012)170].

Abstract

Abstract: Objective To investigate the effect of activation of CB1 on J774A.1 migration and to know whether HuR is involved in the process. Methods Immunofluorescence and agarose gel electrophoresis were used to detect the expression of cannabinoid receptor 1 (CB1) and human antigen R (HuR). Boyden chamber was used for cell migration assay. HuR siRNA transfection was used to determine whether ACEA(CB1 agonist) induced-migration depended on HuR. In order to observe its underlying mechanisms, HuR subcellular localization was assayed by Western blotting of cellular fractions from J774A.1 stimulated with ACEA.The effects of HuR siRNA on CB1 mRNA and protein levels in J774A.1 stimulated with ACEA was assayed by Western blotting. Results In this study, we present evidence that J774A.1 significantly expressed HuR and CB1; the treatment of ACEA caused a increase in J774A.1 migration(P<0.01), and pharmacological inhibition of CB1 markedly attenuated ACEA-induced migration; ACEA-induced J774A.1 migration depended on HuR. Furthermore, we demonstrated ACEA increased HuR cytoplasmic translocation, and HuR regulated the expression of CB1. Conclusion The activation of CB1 induces J774A.1 migration and HuR is involved in the process.

Key words: human antigen R, cannabinoid receptor 1, cell migration

CLC Number:

Zhao Zhongxin, Chang Na, Ge Jingjing, Tian Lei, Li Liying. Mouse monocyte/macrophage cell line J774A.1 migration induced by the activation of CB1 depends on HuR[J]. Journal of Capital Medical University, 2016, 37(1): 76-82.

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Mouse monocyte/macrophage cell line J774A.1 migration induced by the activation of CB1 depends on HuR

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