Journal of Capital Medical University ›› 2012, Vol. 33 ›› Issue (3): 371-376.doi: 10.3969/j.issn.1006-7795.2012.03.018

• 基础研究 • Previous Articles     Next Articles

Effect of the cholinergic anti-inflammatory pathway on acute lung injury induced by oleic acid and lipopolysaccharide in rats

ZHENG Hui1, XU Shao-fa2, JIA Hong-yan3, GU Shu-xiang3   

  1. 1. Department of Anesthesiology, Beijing Chest Hospital, Capital Medical University, Beijing 101149, China;2. Department of Thoracic Surgery, Beijing Chest Hospital, Capital Medical University, Beijing 101149, China;3. Molecular Biology Laboratory of Tuberculosis, Beijing Chest Hospital, Capital Medical University, Beijing 101149, China
  • Received:2012-03-15 Revised:1900-01-01 Online:2012-06-21 Published:2012-06-21

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Abstract: Objective To investigate the effect of the cholinergic anti-inflammatory pathway(CAP) on the acute lung injury(ALI) induced by oleic acid and lipopolysaccharide in rats. Methods Twenty-four male Wistar rats were randomly divided into four groups(n=6). 1Control group: saline, 10 mL·kg-1, intraperitoneally, 2ALI group: oleic acid was administrated with 0.15 mL·kg-1 intravenously 30 min after intraperitoneal injection of lipopolysaccharide (LPS) with 10 mg·kg-1, 3stimulation (ST) group: electrical stimulation(5V, 2 ms, 1Hz) of the right cervical vagus nerve for 10 min before and after LPS and OA administration, 4tetrahydroaminoacridine (THA) group: tetrahydroaminoacridine was given at 1.5 mg·kg-1 intravenously before LPS and OA administration. Blood and tissue samples were collected 130 minutes after the first drug injection in all groups. TLR2 and TLR4 mRNA, NF-κBp65, MPO of lung tissue and IL-6 of serum were measured respectively. The blood gas analysis, wet weight to dry weight ratio (W/D) of right lung, and pathological changes were also observed. Results As compared with group C, the pH and PaO2 decreased markedly in group ALI while the histopathologic changes of lung tissue showed alveolar hemorrhage, necrosis and proteinaceous alveolar edema. W/D ratio, the activity of MPO, NF-κB expression and the level of serum IL-6 significantly increased. As compared with group C, the expressions of the TLR2 and TLR4 mRNA were markedly increased in other three groups, but no significant change was found among these three groups. As compared with group ALI, pH and PaO2 markedly increased in group ST and group THA as well as PaCO2 decreased. The lung tissue showed slight pathological changes. W/D ratio and the activity of MPO were significantly reduced. The expression of NF-κB p65 protein and the amount of serum IL-6 markedly decreased. Conclusions Electrical stimulation of the vagus nerve or administration of THA intravenously could protect the lung against ALI induced by LPS and OA via activating cholinergic anti-inflammatory pathway; the CAP can inhibit the activation of NF-κB and reduce the production of proinflammatory cytokine but had no effect on the expression of TLRs.

Key words: cholinergic anti-inflammatory pathway, acute lung injury, Toll-like receptors

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