Application of ApoC3 transgenic mice in acute pancreatitis with severe hypertrig-lyceridemia

doi:10.3969/j.issn.1006-7795.2020.03.018

Abstract

Abstract: Objective To establish acute pancreatitis mouse model with severe hypertriglyceridemia. Methods Wild type and apolipoprotein C3 (ApoC3) transgenic mice were used to induce acute pancreatitis with caerulein. Plasma levels of lipids, amylase activity and pancreatic pathology were detected. The pancreatic pathological changes were also evaluated by administrating antioxidant terbiary-butylhydroquinone (tBHQ). Results ApoC3 transgenic mice demonstrated significantly higher plasma levels of triglyceride, and aggravated pancreatic pathological injury after inducing acute pancreatitis. Amylase activity in ApoC3 transgenic mice increased at 9h and 12h. The treatment of tBHQ did not improve pancreatic pathology in ApoC3 transgenic mice. Conclusions ApoC3 transgenic mice showed increased plasma amylase activity, and significantly aggravated pancreatic pathological injury after inducing acute pancreatitis. These were similar to pathological changes of pancreas in clinical patients with acute pancreatitis in severe hypertriglyceridemia. ApoC3 transgenic mice could be used as a mouse model for studying pathological mechanism of acute pancreatitis in severe hypertriglyceridemia.

Key words: severe hypertriglyceridemia, acute pancreatitis, apolipoprotein C3 (ApoC3) transgenic, triglyceride, terbiary-butylhydroquinone (tBHQ)

CLC Number:

Liu Xin, Xu Youqing, Cui Chunying, Zhao Zhigang. Application of ApoC3 transgenic mice in acute pancreatitis with severe hypertrig-lyceridemia[J]. Journal of Capital Medical University, 2020, 41(3): 421-427.

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URL: https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/10.3969/j.issn.1006-7795.2020.03.018

https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/Y2020/V41/I3/421

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