Activation of protein phosphatase 2A alleviates α-synuclein-induced apoptosis of SK-N-SH cells

doi:10.3969/j.issn.1006-7795.2013.06.011

Journal of Capital Medical University ›› 2013, Vol. 34 ›› Issue (6): 835-839.doi: 10.3969/j.issn.1006-7795.2013.06.011

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Activation of protein phosphatase 2A alleviates α-synuclein-induced apoptosis of SK-N-SH cells

YANG Weiwei¹, YANG Hui², YU Shun¹

1. Department of Neurobiology, Xuanwu Hospital, Capital Medical University, Laboratory of Molecular Diagnosis, Beijing Geriatric Medical Research Center, Key Laboratory of Neurodegenerative Disease, Ministry of Education, Beijing 100053, China;
2. Department of Neurobiology, School of Basic Medical Sciences, Beijing Insititute for Brain Disorders, Capital Medical University, Beijing 100069, China

Received:2013-10-18 Online:2013-12-21 Published:2013-12-13
Supported by:
This study was supported by the National Basic Research Program of China(2011CB504102，2012CB722407);National Natural Science Foundation of China(30970940);Key Program of Natural Science Foundation of Beijing(7131001).

Abstract

Abstract:

Objective To explore the role of protein phosphatase 2A(PP2A) activity in α-Syn-induced cell apoptosis. Methods SK-N-SH cells were transiently transfected with either myc blank vectors or the myc vectors with α-Syn gene. The expression of α-Syn gene were indentified by immunofluorescence. The PP2A phosphorylation levels and activity were investigated via Western blotting and Cell PP2A Colorimetric Assay Kit, respectively. TUNEL staining and MTT assay were used to evaluate the cell death. Results Compared with control group, α-Syn overexpression in SK-N-SH cells led to phosphorylation of PP2A(high pPP2Ac levels) and inhibition of the enzyme activity. α-Syn overexpression also resulted in the enhancement of apoptotic cells and decrease of cell viable cells. C₂-ceramide, the PP2A activator attenuated α-Syn-induced cell death. Conclusion α-Syn enhanced pPP2Ac levels, and inhibited PP2A activity. Activation of endogenous PP2A alleviates α-Syn-induced apoptosis of SK-N-SH cells.

Key words: α-Synuclein(α-Syn), protein phosphatase 2A(PP2A), Parkinson's disease(PD), apoptosis

CLC Number:

R742.5

YANG Weiwei, YANG Hui, YU Shun. Activation of protein phosphatase 2A alleviates α-synuclein-induced apoptosis of SK-N-SH cells[J]. Journal of Capital Medical University, 2013, 34(6): 835-839.

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Activation of protein phosphatase 2A alleviates α-synuclein-induced apoptosis of SK-N-SH cells

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