A method for evaluation of α-synuclein oligomer formation in plasma of patients with Parkinson’s disease

doi:10.3969/j.issn.1006-7795.2013.06.010

Journal of Capital Medical University ›› 2013, Vol. 34 ›› Issue (6): 830-834.doi: 10.3969/j.issn.1006-7795.2013.06.010

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A method for evaluation of α-synuclein oligomer formation in plasma of patients with Parkinson’s disease

LI Xin^1,2, YANG Weiwei^1,2, LI Yan^1,2, YU Shun^1,2

1. Department of Neurobiology, Xuanwu Hospital, Capital Medical University, Key Laboratory for Neurodegenerative Diseases, Ministry of Education, Beijing 100053, China;
2. Beijing Geriatric Medical and Clinical Center, Beijing 100053, China

Received:2013-10-18 Online:2013-12-21 Published:2013-12-13
Supported by:
This study was supported by the National Natural Science Foundation of China(81071014);National Basic Research and Development Program of China(2011CB504101);Capital Health Research and Development Project(SHOUFA 2011-1001-01);National Science and Technology Support Program(2012BAI10B03);Natural Science Foundation of Beijing(7102076).

Abstract

Abstract:

Objective To establish a method for evaluation of exogenous α-synuclein(α-Syn) oligomer formation in plasma of patients with Parkinson's disease(PD). Methods An ELISA method was developed by using the 3D5 mouse anti-human α-Syn monoclonal antibody. Various concentrations of α-Syn(200, 100, 50, 25, 12.5 μmol/L) was incubated(37℃, 280 r/min) in PD and control sera for 24, 48, 72, and 96 h. The amounts of α-Syn oligomers formed in the sera were measured using the established ELISA method. Results The ELISA method established specifically detected α-Syn oligmers with no reaction to α-Syn monomers. The best differentiation between the α-Syn oligomer formation in PD and control sera was observed when the recombinant α-Syn concentration was 100 μmol/L, with the incubation time being 48 h and the plasma diluted to less than 1/3. Conclusion A method for evaluation of α-Syn oligomer formation in the sera of PD patients and normal controls was established, which can differentiate the potential pathological changes of PD.

Key words: Parkinson’s disease, α-synuclein, plasma, oligomer

CLC Number:

R338

LI Xin, YANG Weiwei, LI Yan, YU Shun. A method for evaluation of α-synuclein oligomer formation in plasma of patients with Parkinson’s disease[J]. Journal of Capital Medical University, 2013, 34(6): 830-834.

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A method for evaluation of α-synuclein oligomer formation in plasma of patients with Parkinson’s disease

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